Disease relevance of IPNVsAgp2

• The nucleotide sequence of infectious pancreatic necrosis virus (IPNV) dsRNA segment A reveals one large ORF encoding a precursor polyprotein [1].
• The proteolytic cascade involved in processing the polyprotein, and in the final maturation of pVP2 (the precursor of VP2), has recently been shown to generate VP2 and four structural peptides in infectious bursal disease virus and blotched snakehead virus [2].
• The capsid of birnaviruses contains two proteins, VP2 and VP3, which derive from the processing of a large polyprotein, NH(2)-pVP2-VP4-VP3-COOH [2].
• Hitherto, neither the PP nor the 17-kDa polypeptide have been identified in infected cells, and the NS (nonstructural) polypeptide was thought not to be part of the virion [3].
• Using recombinant baculoviruses expressing VP1 and PP as markers, the PP could be unambiguously identified in Western blots of infected fish-cell lysates and in purified IPNV [3].

High impact information on IPNVsAgp2

• Synthesis of the infectious pancreatic necrosis virus polyprotein, detection of a virus-encoded protease, and fine structure mapping of genome segment A coding regions [4].
• The larger segment contains a small ORF partly overlapping the 5' end of the polyprotein reading frame [5].
• Sequence homology of OH-IBDV with the most distant serotype 1 IBDV at the nucleotide level is 83.1%, and the amino acid sequence homology of the polyprotein is 89.6% [6].
• Genome Segment A of the Sp serotype is 3097 nucleotides long and contains a major open reading frame (ORF) encoding a polyprotein of 972 amino acids, which initiates at the second in-frame start codon at Position 119 [7].
• A high level of protection was induced only by the plasmid combination that contained a plasmid expressing all the large ORF polyprotein [8].

Biological context of IPNVsAgp2

• Comparison with the Jasper-IPNV polyprotein reveals levels of amino acid sequence homology of about 40% in VP2, 32% in VP3 and 21% in VP4 [9].

Associations of IPNVsAgp2 with chemical compounds

• In addition, Segment A also encodes a 15 kDa arginine-rich non-structural protein from a small ORF, preceding and partially overlapping the polyprotein ORF, which is truncated to 12 kDa in the virulent Sp122 strain [7].

References