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Gene Review:

mutT - nucleoside triphosphate pyrophosphohydrolase

Escherichia coli O157:H7 str. EDL933

Harris, S.L. et al., Yoon, S.H. et al., Sakai, A. et al., Schaaper, R.M. et al., Sakumi, K. et al.

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Disease relevance of mutT

When the human cDNA was expressed in E. coli mutT- mutant cells, there was a significant amount of 8-oxo-dGTPase activity [1].
The mechanism of the Escherichia coli mutT mutator effect was investigated using single-stranded phage as a mutational target [2].

High impact information on mutT

Strong mutator phenotypes of cells defective in both mutM and mutY genes or ones lacking mutT gene were completely suppressed under the anaerobic condition, indicative of an absence of hydroxyl radicals in the cells [3].
Such mutants were isolated by using mutT mutagenesis and an enrichment procedure devised to favor the growth of individuals that could form a pellicle in static broth containing alpha-methylmannoside, an inhibitor of erythrocyte binding and pellicle formation [4].
However, interestingly the presence of MutY increases the mutation frequency in mutT- background because of MutY removes adenine (A) from 8-oxoG:A which arises from the misincorporation of 8-oxoG against A during DNA replication [5].

References

Cloning and expression of cDNA for a human enzyme that hydrolyzes 8-oxo-dGTP, a mutagenic substrate for DNA synthesis. Sakumi, K., Furuichi, M., Tsuzuki, T., Kakuma, T., Kawabata, S., Maki, H., Sekiguchi, M. J. Biol. Chem. (1993) [Pubmed]
Escherichia coli mutT mutator effect during in vitro DNA synthesis. Enhanced A.G replicational errors. Schaaper, R.M., Dunn, R.L. J. Biol. Chem. (1987) [Pubmed]
Impact of reactive oxygen species on spontaneous mutagenesis in Escherichia coli. Sakai, A., Nakanishi, M., Yoshiyama, K., Maki, H. Genes Cells (2006) [Pubmed]
Isolation and characterization of mutants with lesions affecting pellicle formation and erythrocyte agglutination by type 1 piliated Escherichia coli. Harris, S.L., Elliott, D.A., Blake, M.C., Must, L.M., Messenger, M., Orndorff, P.E. J. Bacteriol. (1990) [Pubmed]
MutY is down-regulated by oxidative stress in E. coli. Yoon, S.H., Lee, H.S., Choi, J.Y., Kang, H.K., Lee, J.J., Hyun, J.W., Choi, J., Ye, S.K., Chung, M.H. Free Radic. Res. (2003) [Pubmed]

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