Disease relevance of ureF

• Using the best-studied urease system, in which the Klebsiella aerogenes genes are expressed in Escherichia coli, a translational fusion of ureE and ureF was generated [1].
• Slow and partial activation of urease apoprotein was observed after addition of nickel ion to extracts of Escherichia coli cells bearing a plasmid containing the K. aerogenes urease gene cluster or derivatives of this plasmid with deletions in ureE, ureF, or ureG [2].

High impact information on ureF

• Potential roles for ureE, ureF, and ureG were explored by deleting these accessory genes from the operon [3].
• Possible termination sites were found downstream of ureD, ureC, and ureF [4].

Biological context of ureF

• Uniquely, the ureE and ureF genes are fused to form a hybrid protein, UreEF, which may result in tighter coordination of the putative functions of the individual accessory genes, nickel donation to the urease active site, and prevention of nickel incorporation until correct formation of the active site, respectively [5].

Other interactions of ureF

• Thus, one or more of the ureE, ureF, or ureG gene products are involved in nickel incorporation into urease [3].

References