Disease relevance of ureC

• We have investigated the distribution of ureC in 202 Shiga toxin-producing E. coli (STEC) strains from Austria belonging to 61 different serotypes [1].
• To study this interaction, strains of Proteus mirabilis and P. vulgaris and their isogenic hemolysin-negative (hpmA) or isogenic urease-negative (ureC) constructs were overlaid onto cultures of human renal proximal tubular epithelial cells (HRPTEC) isolated from kidneys obtained by immediate autopsy [2].

High impact information on ureC

• We disrupted each of the ureR, ureC, and nikD genes in TH3996 by homologous recombination and analyzed the phenotype of the mutants [3].
• By using as a probe a 0.6-kpb internal fragment of the S. salivarius 57.I ureC gene, two clones from subgenomic libraries of S. salivarius 57.I in an Escherichia coli plasmid vector were identified [4].
• The structural genes ureA, ureB, and ureC encode the 11-, 12-, and 61-kDa subunits, respectively [5].
• The ureC, ureD, and ureI genes are not essential for urease expression in E. coli, although they belong to the urease gene cluster [6].
• The three subunits of the nickel-containing enzyme are encoded by ureA, ureB, and ureC based on protein structural studies and sequence homology to jack bean urease [7].
• Neutral pH environments, excess carbohydrate or limited nitrogen source could promote ureC expression [8]. Similar regulation was been observed in of Actinomyces naeslundii. [9][10]

Biological context of ureC

• A component of the ure gene cluster in E. coli, ureC, encodes a subunit of urease [1].

Associations of ureC with chemical compounds

• A cassette containing the Tn903 kanamycin resistance determinant (km(r)) and the sacB gene expressed from the A. pleuropneumoniae omlA promoter was introduced by homologous recombination into the ureC gene of A. pleuropneumoniae [11].
• Disruption of ureC prevented the utilization of urea as a nitrogen source by C. glutamicum [12].

Other interactions of ureC

• Possible termination sites were found downstream of ureD, ureC, and ureF [13].
• The ureC gene was more frequent among STEC isolates harbouring eae than among those lacking eae (p < 0.0001) [14].

Analytical, diagnostic and therapeutic context of ureC

• Although the strains possessed the ureC gene, as shown by PCR, this gene did not appear to contribute to acid resistance under the conditions tested [15].

References