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Gene Review:

infA - translation initiation factor IF-1

Escherichia coli O157:H7 str. EDL933

Cummings, H.S. et al., Croitoru, V. et al., Gualerzi, C.O. et al., Shen, L. et al., Ko, J.H. et al., et al.

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Disease relevance of infA

Cell viability is maintained only when infA is expressed in trans from a plasmid, thereby demonstrating that IF1 is essential for cell growth in Escherichia coli [1].

High impact information on infA

In this study, we found that infA mRNA was greatly increased, and that this increase resulted from transcriptional activation of P1, not P2, during cold shock although stability of transcripts from both promoters concomitantly increased [2].
Genes involved in protein biosynthesis, encoding the ribosome-associated protein RpsV (sra) and the initiation factor IF-1 (infA), were also induced in an rpoS-dependent fashion [3].
To elucidate the function of IF1 and determine if the protein is essential for cell growth, the chromosomal copy of infA was disrupted [1].
Translation initiation factor IF1 is essential for cell viability in Escherichia coli [1].
Some of the mutated infA genes were successfully recombined into the chromosome thereby replacing the wild-type infA+ allele [4].

Chemical compound and disease context of infA

Starting from a synthetic modular gene (infA) encoding Escherichia coli translation initiation factor IF1, we have constructed mutants in which amino acids are deleted from the carboxyl terminus or in which His29 or His34 are replaced by Tyr or Asp residues [5].

Anatomical context of infA

Interaction of Escherichia coli translation-initiation factor IF-1 with ribosomes [6].

Associations of infA with chemical compounds

The purpose of our study was to examine the expression of a cluster of genes that are required for translation in C. trachomatis serovar F: infA (encoding Initiation Factor 1), tRNA(Thr), tuf (encoding Elongation Factor Tu), and tRNA(Trp) [7].

Analytical, diagnostic and therapeutic context of infA

Because IF1 is essential for cell viability and no mutant selection has so far been described, the infA gene in a plasmid was mutated by site-directed mutagenesis in a strain with a chromosomal infA+ gene, followed by deletion of this infA+ gene [4].
The interaction between Escherichia coli translation-initiation factor IF-1 and ribosomes was studied in binding experiments by Airfuge centrifugation [6].

References

Translation initiation factor IF1 is essential for cell viability in Escherichia coli. Cummings, H.S., Hershey, J.W. J. Bacteriol. (1994) [Pubmed]
Differential promoter usage of infA in response to cold shock in Escherichia coli. Ko, J.H., Lee, S.J., Cho, B., Lee, Y. FEBS Lett. (2006) [Pubmed]
SigmaS-dependent gene expression at the onset of stationary phase in Escherichia coli: function of sigmaS-dependent genes and identification of their promoter sequences. Lacour, S., Landini, P. J. Bacteriol. (2004) [Pubmed]
Generation and characterization of functional mutants in the translation initiation factor IF1 of Escherichia coli. Croitoru, V., Bucheli-Witschel, M., Hägg, P., Abdulkarim, F., Isaksson, L.A. Eur. J. Biochem. (2004) [Pubmed]
Site-directed mutagenesis of Escherichia coli translation initiation factor IF1. Identification of the amino acid involved in its ribosomal binding and recycling. Gualerzi, C.O., Spurio, R., La Teana, A., Calogero, R., Celano, B., Pon, C.L. Protein Eng. (1989) [Pubmed]
Interaction of Escherichia coli translation-initiation factor IF-1 with ribosomes. Celano, B., Pawlik, R.T., Gualerzi, C.O. Eur. J. Biochem. (1988) [Pubmed]
Identification and characterization of promoters regulating tuf expression in Chlamydia trachomatis serovar F. Shen, L., Shi, Y., Douglas, A.L., Hatch, T.P., O'Connell, C.M., Chen, J.M., Zhang, Y.X. Arch. Biochem. Biophys. (2000) [Pubmed]

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