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Gene Review: 

nodI  -  nodulation protein NodI

Sinorhizobium fredii NGR234

 
 
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Disease relevance of nodI

  • The nodI and nodJ gene products shared about 70% sequence similarity with the corresponding Rhizobium leguminosarum bv. viciae proteins; NodI belongs to the family of ATP-binding proteins that are constituents of bacterial binding protein-dependent transport systems [1].
  • The predicted amino acid sequence of the nodI gene shows considerable homology to inner-membrane-located gene products involved in active transport systems in Escherichia coli and Salmonella typhimurium [2].
 

High impact information on nodI

  • Nucleotide sequence of Rhizobium loti nodI [3].
  • By contrast, mutations in the nodI and nodJ genes produced a Nod+ phenotype with a reduced number of nodules on the temperate hosts [4].
  • The role of the nodI and nodJ genes in the transport of Nod metabolites in Rhizobium etli [5].
  • By using plasmids in which the nodI and nodJ genes are cloned separately under control of a flavonoid-inducible promoter, it was shown that both genes are needed for a wild-type level of LCO secretion [6].
  • By using immunoblots, it was found that the levels of NodA, NodI, NodE, and NodO proteins were reduced at least 14-fold in bacteriods compared with cultured cells, whereas NodD protein was reduced only 3-fold [7].
 

Biological context of nodI

  • One of these transposons was cloned along with flanking DNA; the flanking DNA was sequenced (858 base pairs total), and the predicted amino acid sequence showed homology with a family of proteins including the Rhizobium leguminosarum nodI gene, many of whose members bind ATP and have been implicated in active transport systems [8].
  • Genes corresponding to the two open reading frames identified are named nodI and nodJ [2].
  • By the use of antibodies raised against a fusion protein of lacZ'-nodI (produced in Escherichia coli) which specifically react with NodI protein, it was shown that in wild-type Rhizobium leguminosarum biovar viciae NodI protein (i) is recovered with the cytoplasmic membrane fraction and (ii) is translated as part of the nodABCIJ operon [9].

References

  1. Identification of nodS and nodU, two inducible genes inserted between the Bradyrhizobium japonicum nodYABC and nodIJ genes. Göttfert, M., Hitz, S., Hennecke, H. Mol. Plant Microbe Interact. (1990) [Pubmed]
  2. The nodI gene product of Rhizobium leguminosarum is closely related to ATP-binding bacterial transport proteins; nucleotide sequence analysis of the nodI and nodJ genes. Evans, I.J., Downie, J.A. Gene (1986) [Pubmed]
  3. Nucleotide sequence of Rhizobium loti nodI. Young, C., Collins-Emerson, J.M., Terzaghi, E.A., Scott, D.B. Nucleic Acids Res. (1990) [Pubmed]
  4. Sequence and mutational analysis of the common nodBCIJ region of Rhizobium sp. (Oxytropis arctobia) strain N33, a nitrogen-fixing microsymbiont of both arctic and temperate legumes. Cloutier, J., Laberge, S., Prévost, D., Antoun, H. Mol. Plant Microbe Interact. (1996) [Pubmed]
  5. The role of the nodI and nodJ genes in the transport of Nod metabolites in Rhizobium etli. Cárdenas, L., Domínguez, J., Santana, O., Quinto, C. Gene (1996) [Pubmed]
  6. Rhizobium NodI and NodJ proteins play a role in the efficiency of secretion of lipochitin oligosaccharides. Spaink, H.P., Wijfjes, A.H., Lugtenberg, B.J. J. Bacteriol. (1995) [Pubmed]
  7. Suppression of nodulation gene expression in bacteroids of Rhizobium leguminosarum biovar viciae. Schlaman, H.R., Horvath, B., Vijgenboom, E., Okker, R.J., Lugtenberg, B.J. J. Bacteriol. (1991) [Pubmed]
  8. Transcriptional regulation of the virA and virG genes of Agrobacterium tumefaciens. Winans, S.C., Kerstetter, R.A., Nester, E.W. J. Bacteriol. (1988) [Pubmed]
  9. Subcellular localization of the Rhizobium leguminosarum nodI gene product. Schlaman, H.R., Okker, R.J., Lugtenberg, B.J. J. Bacteriol. (1990) [Pubmed]
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