The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Gene Review

nodI  -  nodulation protein NodI

Sinorhizobium fredii NGR234

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of nodI

  • The nodI and nodJ gene products shared about 70% sequence similarity with the corresponding Rhizobium leguminosarum bv. viciae proteins; NodI belongs to the family of ATP-binding proteins that are constituents of bacterial binding protein-dependent transport systems [1].
  • The predicted amino acid sequence of the nodI gene shows considerable homology to inner-membrane-located gene products involved in active transport systems in Escherichia coli and Salmonella typhimurium [2].

High impact information on nodI

  • Nucleotide sequence of Rhizobium loti nodI [3].
  • By contrast, mutations in the nodI and nodJ genes produced a Nod+ phenotype with a reduced number of nodules on the temperate hosts [4].
  • The role of the nodI and nodJ genes in the transport of Nod metabolites in Rhizobium etli [5].
  • By using plasmids in which the nodI and nodJ genes are cloned separately under control of a flavonoid-inducible promoter, it was shown that both genes are needed for a wild-type level of LCO secretion [6].
  • By using immunoblots, it was found that the levels of NodA, NodI, NodE, and NodO proteins were reduced at least 14-fold in bacteriods compared with cultured cells, whereas NodD protein was reduced only 3-fold [7].

Biological context of nodI

  • One of these transposons was cloned along with flanking DNA; the flanking DNA was sequenced (858 base pairs total), and the predicted amino acid sequence showed homology with a family of proteins including the Rhizobium leguminosarum nodI gene, many of whose members bind ATP and have been implicated in active transport systems [8].
  • Genes corresponding to the two open reading frames identified are named nodI and nodJ [2].
  • By the use of antibodies raised against a fusion protein of lacZ'-nodI (produced in Escherichia coli) which specifically react with NodI protein, it was shown that in wild-type Rhizobium leguminosarum biovar viciae NodI protein (i) is recovered with the cytoplasmic membrane fraction and (ii) is translated as part of the nodABCIJ operon [9].


  1. Identification of nodS and nodU, two inducible genes inserted between the Bradyrhizobium japonicum nodYABC and nodIJ genes. Göttfert, M., Hitz, S., Hennecke, H. Mol. Plant Microbe Interact. (1990) [Pubmed]
  2. The nodI gene product of Rhizobium leguminosarum is closely related to ATP-binding bacterial transport proteins; nucleotide sequence analysis of the nodI and nodJ genes. Evans, I.J., Downie, J.A. Gene (1986) [Pubmed]
  3. Nucleotide sequence of Rhizobium loti nodI. Young, C., Collins-Emerson, J.M., Terzaghi, E.A., Scott, D.B. Nucleic Acids Res. (1990) [Pubmed]
  4. Sequence and mutational analysis of the common nodBCIJ region of Rhizobium sp. (Oxytropis arctobia) strain N33, a nitrogen-fixing microsymbiont of both arctic and temperate legumes. Cloutier, J., Laberge, S., Prévost, D., Antoun, H. Mol. Plant Microbe Interact. (1996) [Pubmed]
  5. The role of the nodI and nodJ genes in the transport of Nod metabolites in Rhizobium etli. Cárdenas, L., Domínguez, J., Santana, O., Quinto, C. Gene (1996) [Pubmed]
  6. Rhizobium NodI and NodJ proteins play a role in the efficiency of secretion of lipochitin oligosaccharides. Spaink, H.P., Wijfjes, A.H., Lugtenberg, B.J. J. Bacteriol. (1995) [Pubmed]
  7. Suppression of nodulation gene expression in bacteroids of Rhizobium leguminosarum biovar viciae. Schlaman, H.R., Horvath, B., Vijgenboom, E., Okker, R.J., Lugtenberg, B.J. J. Bacteriol. (1991) [Pubmed]
  8. Transcriptional regulation of the virA and virG genes of Agrobacterium tumefaciens. Winans, S.C., Kerstetter, R.A., Nester, E.W. J. Bacteriol. (1988) [Pubmed]
  9. Subcellular localization of the Rhizobium leguminosarum nodI gene product. Schlaman, H.R., Okker, R.J., Lugtenberg, B.J. J. Bacteriol. (1990) [Pubmed]
WikiGenes - Universities