High impact information on MTFR1

• Chondrocyte protein with a poly-proline region (CHPPR) is a novel mitochondrial protein and promotes mitochondrial fission [1].
• Conventional fluorescence and confocal localization of CHPPR shows that CHPPR is sorted to mitochondria [1].
• We have recently identified a chondrocyte protein with a poly-proline region, referred to as CHPPR, and showed that this protein is expressed intracellularly in chick embryo chondrocytes [1].
• Furthermore, immunoelectron microscopy of CHPPR transfected cells demonstrates that this protein is mostly associated with the mitochondrial inner membranes [1].
• Since cartilage expresses high levels of CHPPR mRNA when compared to other tissues and because CHPPR is associated with late stages of chondrocyte differentiation, we have investigated mitochondrial morphology in hypertrophic chondrocytes by MitoTracker Orange labeling [1].

Biological context of MTFR1

• The CHPPR cDNA has a complete open reading frame coding for a polypeptide with a calculated mass of 35.6 kDa containing a proline-rich region with a PPLP motif (single-letter amino acid code) [2].

Anatomical context of MTFR1

• Careful analysis of CHPPR expressing cells reveals, instead of the regular mitochondrial tubular network, the presence of a number of small spheroid mitochondria [1].
• OBJECTIVE: The minimal tonic firing rates (the lowest firing rates at which motor units fire regularly; MTFR) of single motor units (SMUs) within the lateral pterygoid muscle have not been widely investigated [3].

Regulatory relationships of MTFR1

• The Parathyroid Hormone peptide [PTH (1-34)] enhances accumulation of CHPPR mRNA in cultured chondrocytes [2].

Analytical, diagnostic and therapeutic context of MTFR1

• We demonstrate by Western blot analysis that two CHPPR isoforms are detected in the cell lysates from cultured chondrocytes when they are not in the culture medium; furthermore, we find that the CHPPR gene is expressed in vivo by chick embryo chondrocytes at higher levels in the prehypertrophic and hypertrophic zones [2].

References