Disease relevance of 5-diethylamino-2-[4-(dimethylsulfamoyl)phenyl]diazenyl-N-(2-pyridin-1-ylethyl)benzenesulfonamide

• Agent Orange and birth defects (continued) [1].
• Thoracic soft-tissue sarcoma in Vietnam veterans exposed to Agent Orange [2].
• Lead poisoning among low-income children in Orange County, California. A need for regionally differentiated policy [3].
• Agent Orange and spontaneous abortions [4].
• Two chemoattractants for human monocytes were purified to apparent homogeneity from the culture supernatant of a glioma cell line (U-105MG) by sequential chromatography on Orange A-Sepharose, an HPLC cation exchanger, and a reverse phase HPLC column [5].

Psychiatry related information on 5-diethylamino-2-[4-(dimethylsulfamoyl)phenyl]diazenyl-N-(2-pyridin-1-ylethyl)benzenesulfonamide

• SETTING: Alzheimer's Disease Research Center Consortium of Los Angeles and Orange Counties, California. SUBJECTS: Volunteers, recruited from the community or clinic referrals, who met clinical criteria for AD (n = 159) or VD (n = 117) or met criteria for control status without dementia (n = 134) [6].
• Some took a psychological form (exhaustion, combat fatigue, combat stress reaction and post-traumatic stress disorder), while others were characterized by medically unexplained symptoms (soldier's heart, effort syndrome, shell shock, non-ulcer dyspepsia, effects of Agent Orange and Gulf War Syndrome) [7].
• Two strategies for preventing the onset of alcohol abuse, and marijuana and cigarette use were tested in junior high schools in Los Angeles and Orange Counties, California. The first strategy taught skills to refuse substance use offers [8].
• The authors believe that the mental health worker program of the Orange County, California, community mental health program is developed and stable encough that the initial steps of an economic analysis are possible [9].
• Carcinogenicity and teratogenicity vs. psychogenicity: psychological characteristics associated with self-reported Agent Orange exposure among Vietnam combat veterans who seek treatment for substance abuse [10].

High impact information on 5-diethylamino-2-[4-(dimethylsulfamoyl)phenyl]diazenyl-N-(2-pyridin-1-ylethyl)benzenesulfonamide

• CONCLUSIONS--These data suggest that lead poisoning is not a major public health problem among Orange County children and detection of infrequent cases would involve a high cost per child [3].
• PARTICIPANTS--A total of 5115 Orange County children between 12 and 72 months of age using EPSDT services from March through December 1992 [3].
• Dioxins and dibenzofurans in blood and adipose tissue of Agent Orange-exposed Vietnam veterans and matched controls [11].
• The Orange (Or) gene mutation in cauliflower (Brassica oleracea var botrytis) confers the accumulation of high levels of beta-carotene in various tissues normally devoid of carotenoids [12].
• We show that the Orange domain is an important functional domain that confers specificity among members of the Hairy/E(SPL) family [13].

Chemical compound and disease context of 5-diethylamino-2-[4-(dimethylsulfamoyl)phenyl]diazenyl-N-(2-pyridin-1-ylethyl)benzenesulfonamide

• The fluorescence technique described here utilizes the electrostatic interaction between the polyanionic sites of glycosaminoglycans and the cationic dye Acridine Orange to analyse urinary glycosaminoglycans from patients suffering from mucopolysaccharidoses [14].
• Tear proteins of nonstimulated tears of 29 patients (healthy subjects, n = 8; dry-eye syndrome patients, n = 12; diabetic dry-eye patients, n = 9) were electrophoretically separated and stained by SYPRO Orange, followed by Coomassie blue staining [15].
• 2. Glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides was purified approx. 56-fold in a two-step tandem chromatographic system using Matrex Gel Purple A followed by Matrex Gel Orange B chromatography to a specific activity of 228 units/mg of protein in a final yield of 73% [16].
• Long-term follow-up of short intensive multiagent chemotherapy without high-dose methotrexate ('Orange') in children with advanced non-lymphoblastic non-Hodgkin's lymphoma: a children's cancer group report [17].
• There is conflicting research regarding an association between fetal death and paternal exposure to Agent Orange, a phenoxy herbicide widely used in Vietnam that was contaminated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) [18].

Biological context of 5-diethylamino-2-[4-(dimethylsulfamoyl)phenyl]diazenyl-N-(2-pyridin-1-ylethyl)benzenesulfonamide

• Domain swapping and mutagenesis of the Hairy and E(SPL) proteins show that three evolutionarily conserved domains are required for their function: the bHLH, Orange, and WRPW domains [13].
• The fluorescent mitochondrial probe MitoTracker Orange indicated that cells undergoing apoptosis became TUNEL positive before loss of mitochondrial function [19].
• During oogenesis, the yolk platelets accumulated a constant amount of Acridine Orange, corresponding to a pH of around 5 [20].
• Rhodamine 123 and Nonyl Acridine Orange were used as specific probes to monitor the membrane potential and mass of mitochondria, respectively, by means of flow cytometry [21].
• CONCLUSIONS: Orange juice substantially reduces the bioavailability of celiprolol, but the mechanism of this interaction remains to be resolved [22].

Anatomical context of 5-diethylamino-2-[4-(dimethylsulfamoyl)phenyl]diazenyl-N-(2-pyridin-1-ylethyl)benzenesulfonamide

• We have investigated the interactions with isolated mitochondria and intact cells of chloromethyltetramethylrosamine (CMTMRos), a probe (Mitotracker Orange) that is increasingly used to monitor the mitochondrial membrane potential (Deltapsi(m)) in situ [23].
• Phase-dense vesicles were identified as lysosomes or other acidic vesicles by vital staining with Acridine Orange. After differential centrifugation of thyroid homogenates, calmodulin was measured by radioimmunoassay (RIA) and found in both cytosolic (89%) and membrane vesicle fractions (11%) [24].
• Within 0-30 minutes, fluo-NT accumulated together with its receptor in Acridine Orange-positive, acidic organelles [25].
• Heating the erythrocytes to 50 degrees C, at which temperature denaturation of spectrin and fragmentation of the erythrocytes occur, and subsequently incubating them in Acridine Orange at room temperature, caused an almost maximal rate of particle aggregation within 10-30 s, without haemolysis [26].
• Acridine Orange, used as an in vivo indicator of acidic compartments, stained the secretory vesicles in both wild-type and mutant cells [27].

Associations of 5-diethylamino-2-[4-(dimethylsulfamoyl)phenyl]diazenyl-N-(2-pyridin-1-ylethyl)benzenesulfonamide with other chemical compounds

• In this report, mass spectra generated from human serum or rat liver proteins stained with either colloidal Coomassie blue, Daiichi silver, SYPRO Orange, SYPRO Red, SYPRO Ruby, or SYPRO Tangerine are compared [28].
• ATP-driven acidification of internal compartments of Trypanosoma cruzi epimastigotes was assayed spectrophotometrically with Acridine Orange and cells permeabilized with filipin [29].
• Inorganic pyrophosphate promoted the acidification of an intracellular compartment in permeabilized promastigotes of Leishmania donovani, as measured by Acridine Orange uptake [30].
• Anion-exchange chromatography on Q-Sepharose, followed by adsorption chromatography on columns of Matrex Orange A and Cibacron Blue, have been employed to purify rAFAR2 from rat liver cytosol [31].
• These data provide little or no support for the theory that paternal exposure to Agent Orange and its dioxin contaminant is associated with adverse reproductive outcomes [32].

Gene context of 5-diethylamino-2-[4-(dimethylsulfamoyl)phenyl]diazenyl-N-(2-pyridin-1-ylethyl)benzenesulfonamide

• In contrast, only the bHLH domain was required for the heterodimerization with Hey2, whereas only the Orange domain mediated the interaction between Helt and Hes5 [33].
• The ability of Hes1 to interact with Ptf1-p48 resides within a fragment comprised of the bHLH, Orange and C-terminal domains, and does not require the N-terminal or WRPW elements [34].
• While the overall structure with the bHLH domain, Orange domain, and WRPW motif is similar, the last motif is changed to KPYRPWG in Hey1/2 and absent in HeyL [35].
• We report a novel missense change in BRCA1, 2640 C-->T (R841W), found in 3 cases from a subject group of 305 breast and 79 ovarian cancer cases from Orange County, CA [36].
• In the current study, DNA resequencing/haplotyping was conducted on 136 DRD4 alleles obtained from autism probands, collected from the same geographic population as the prior ADHD probands (Orange County, CA) [37].

Analytical, diagnostic and therapeutic context of 5-diethylamino-2-[4-(dimethylsulfamoyl)phenyl]diazenyl-N-(2-pyridin-1-ylethyl)benzenesulfonamide

• Two containing D&C Orange No. 17 were directly mutagenic [38].
• The protein, purified by affinity chromatography with Amicon Matrex Gel Orange A, had enzyme activity characteristic of aldose reductase (alditol:NADPH+ oxidoreductase, EC 1.1.1.21) [39].
• Isoelectric focusing on a Sephadex G-15 support bed or ampholyte displacement chromatography of Orange A bound material demonstrated inhibitory activity at pH 3.5-4.5 and 6.5-7 [40].
• The Chlase1 cDNA from Valencia Orange (Citrus sinensis cv. Valencia) was obtained by RT-PCR using degenerate primers based on the amino acid sequence of the previously purified protein [41].
• Two suitable DNA binding dyes, SYTOX Orange and TO-PRO-3, were selected and tested for the real-time PCR processes [42].

References