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Gene Review:

NSm - non-structural protein

Groundnut bud necrosis virus

Okuda, M. et al., Cortez, I. et al., Soellick, T. et al., Knierim, D. et al., Storms, M.M. et al., et al.

Lewandowski, Adkins, Okuda, Kato, Hanada, Iwanami,

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Disease relevance of NSm

The movement protein NSm of tomato spotted wilt tospovirus (TSWV): RNA binding, interaction with the TSWV N protein, and identification of interacting plant proteins [1].
To study the molecular basis of NSm function, we expressed the protein in Escherichia coli and investigated protein-protein and protein-RNA interactions of NSm protein in vitro [1].
Spodoptera frugiperda and Trichoplusia ni cells were infected with a recombinant baculovirus expressing the NSm-gene (AcNPV/NSm) [2].

High impact information on NSm

Tubules, characteristic of the NSm protein, were also formed in tobacco protoplasts infected with the TMV-TSWV hybrids [3].
The M RNA segment contains one open reading frame (ORF) encoding 308 amino acids (aa) in the sense orientation and another ORF encoding 1,127 aa in the complementary orientation, which were homologous to the NSm protein and G1/G2 glycoprotein precursor (Gp) protein, respectively [4].
Two ORFs are located on the M RNA (4823 nt) encoding the non-structural (NSm) protein (308 aa) and the viral glycoprotein precursors (Gn/Gc) (1121 aa) separated by an intergenic region of 433 nt [5].
The vRNA ORF codes for the potential cell-to-cell movement (NSm) protein (34.8 kDa) and the vcRNA ORF for the viral glycoprotein (G1/G2) precursor (128.6 kDa) [6].

Chemical compound and disease context of NSm

Multiple sequence alignment of the NSm and G1/G2 precursor proteins of IYSV with those of other tospoviruses, showed highest homologies to Peanut bud necrosis virus (PBNV) and Watermelon silver mottle virus (WSMV) [6].

Biological context of NSm

To analyze subgenomic RNA of the M RNA segment, RNA transcripts corresponding to the NSm and Gp genes were specifically amplified, and the nucleotide sequence of the 5' terminal region was determined [4].

Other interactions of NSm

Sequence analysis of the NSm and Gp transcripts showed that they had a non-viral sequence 12-18 and 10-18 nucleotides long, respectively [4].

References

The movement protein NSm of tomato spotted wilt tospovirus (TSWV): RNA binding, interaction with the TSWV N protein, and identification of interacting plant proteins. Soellick, T., Uhrig, J.F., Bucher, G.L., Kellmann, J.W., Schreier, P.H. Proc. Natl. Acad. Sci. U.S.A. (2000) [Pubmed]
The nonstructural NSm protein of tomato spotted wilt virus induces tubular structures in plant and insect cells. Storms, M.M., Kormelink, R., Peters, D., Van Lent, J.W., Goldbach, R.W. Virology (1995) [Pubmed]
The tubule-forming NSm protein from Tomato spotted wilt virus complements cell-to-cell and long-distance movement of Tobacco mosaic virus hybrids. Lewandowski, D.J., Adkins, S. Virology (2005) [Pubmed]
Nucleotide sequence of melon yellow spot virus M RNA segment and characterization of non-viral sequences in subgenomic RNA. Okuda, M., Kato, K., Hanada, K., Iwanami, T. Arch. Virol. (2006) [Pubmed]
The complete nucleotide sequence of a capsicum chlorosis virus isolate from Lycopersicum esculentum in Thailand. Knierim, D., Blawid, R., Maiss, E. Arch. Virol. (2006) [Pubmed]
Genetic organisation of Iris yellow spot virus M RNA: indications for functional homology between the G(C) glycoproteins of tospoviruses and animal-infecting bunyaviruses. Cortez, I., Aires, A., Pereira, A.M., Goldbach, R., Peters, D., Kormelink, R. Arch. Virol. (2002) [Pubmed]

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