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Disease relevance of Spodoptera


High impact information on Spodoptera

  • Several commercial solvent mixtures commonly used as insecticide carriers in spray formulations increase by more than threefold the microsomal N-demethylation of p-chloro N-methylaniline in midgut preparations of southern army-worm (Spodoptera eridania) larvae exposed orally to the test solvents [6].
  • Heterologous expression of KCO1 in baculovirus-infected insect (Spodoptera frugiperda) cells resulted in outwardly rectifying, K+-selective currents elicited by depolarizing voltage pulses in whole-cell measurements [7].
  • The p10 gene promoter was inactive in human HeLa cells and in uninfected Spodoptera frugiperda insect cells [8].
  • For example, maize seedlings attacked by beet armyworm larvae (Spodoptera exigua) produce a mixture of terpenoid and indole volatiles that serve to attract parasitic wasps [9].
  • We have expressed these five forms of the pol gene in Spodoptera frugiperda SF9 cells and have analyzed for both reverse transcriptase and RNase H activities [10].

Chemical compound and disease context of Spodoptera


Biological context of Spodoptera


Anatomical context of Spodoptera

  • The insect cell line derived from Spodoptera frugiperda (Sf9) does not express the activities of the trifunctional NADP-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase [21].
  • Molecular cloning and characterization of hemolymph 3-dehydroecdysone 3beta-reductase from the cotton leafworm, Spodoptera littoralis. A new member of the third superfamily of oxidoreductases [22].
  • In attempting to isolate and biochemically characterize mammalian proteins capable of regulating various activities of CDC42Hs, we have identified an activity in bovine brain cytosol which effectively inhibits the dissociation of [3H]GDP from the platelet- or the Spodoptera frugiperda-expressed CDC42Hs protein [23].
  • EPR spectroscopy was used to investigate the cytochrome P-450-dependent steroid hydroxylase ecdysone 20-mono-oxygenase of the cotton leafworm (Spodoptera littoralis) and the redox centres associated with membranes from the fat-body mitochondrial fraction [24].
  • In the present study, the newly described fluorescent dye CD-222 was used to monitor extracellular potassium ion concentration and report the effects of these antibiotics on the K+ permeability of the plasma membrane of Spodoptera frugiperda (Sf9) and Choristoneura fumiferana (Cf1) insect cells [25].

Associations of Spodoptera with chemical compounds

  • Infected Spodoptera frugiperda (Sf9) cells secrete immunoreactive hCBG at high levels (16-24 pmol per 10(6) cells per 40 h), and the recombinant protein binds cortisol with an affinity and specificity equivalent to that of human serum-derived hCBG [26].
  • Rabbit brain tryptophan hydroxylase (TPH) has been expressed in insect cells (Spodoptera frugiperda) as a histidine-tagged enzyme [27].
  • The primary product of the prothoracic glands of last instar larvae of Spodoptera littoralis is 3-dehydroecdysone (3DE) [22].
  • Purification, cloning, and bacterial expression of retinol dehydratase from Spodoptera frugiperda [28].
  • Regulation of ecdysteroid signaling: cloning and characterization of ecdysone oxidase: a novel steroid oxidase from the cotton leafworm, Spodoptera littoralis [29].

Gene context of Spodoptera

  • The full-length murine erythropoietin receptor was expressed in Spodoptera frugiperda (Sf9) cells using a recombinant baculovirus vector [30].
  • Expression levels were optimized in Spodoptera frugiperda (Sf9) cells and were in the following order hMOR > hDOR > hKOR [31].
  • Full-length MRP as well as molecules corresponding to either the NH2- or COOH-proximal halves of the protein were expressed individually and in combination in Spodoptera frugiperda Sf21 cells [32].
  • Properties of recombinant mouse thrombospondin 2 expressed in Spodoptera cells [33].
  • The purified brain GDI protein shows little ability to inhibit GDP dissociation from the E. coli-expressed CDC42Hs and is capable of only a very weak inhibition of the dissociation of [35S]guanosine 5'-3-O-(thio)triphosphate (GTP gamma S) from the Spodoptera frugiperda-expressed CDC42 [23].

Analytical, diagnostic and therapeutic context of Spodoptera


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  14. Ligand binding properties of muscarinic acetylcholine receptor subtypes (m1-m5) expressed in baculovirus-infected insect cells. Dong, G.Z., Kameyama, K., Rinken, A., Haga, T. J. Pharmacol. Exp. Ther. (1995) [Pubmed]
  15. 5-lipoxygenase-activating protein is an arachidonate binding protein. Mancini, J.A., Abramovitz, M., Cox, M.E., Wong, E., Charleson, S., Perrier, H., Wang, Z., Prasit, P., Vickers, P.J. FEBS Lett. (1993) [Pubmed]
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  19. The Drosophila DIAP1 protein is required to prevent accumulation of a continuously generated, processed form of the apical caspase DRONC. Muro, I., Hay, B.A., Clem, R.J. J. Biol. Chem. (2002) [Pubmed]
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  21. The NADP-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase is not expressed in Spodoptera frugiperda cells. Tremblay, G.B., Mejia, N.R., MacKenzie, R.E. J. Biol. Chem. (1992) [Pubmed]
  22. Molecular cloning and characterization of hemolymph 3-dehydroecdysone 3beta-reductase from the cotton leafworm, Spodoptera littoralis. A new member of the third superfamily of oxidoreductases. Chen, J.H., Turner, P.C., Rees, H.H. J. Biol. Chem. (1999) [Pubmed]
  23. The identification and characterization of a GDP-dissociation inhibitor (GDI) for the CDC42Hs protein. Leonard, D., Hart, M.J., Platko, J.V., Eva, A., Henzel, W., Evans, T., Cerione, R.A. J. Biol. Chem. (1992) [Pubmed]
  24. EPR spectroscopic characterization of the iron-sulphur proteins and cytochrome P-450 in mitochondria from the insect Spodoptera littoralis (cotton leafworm). Shergill, J.K., Cammack, R., Chen, J.H., Fisher, M.J., Madden, S., Rees, H.H. Biochem. J. (1995) [Pubmed]
  25. Real-time fluorimetric analysis of gramicidin D- and alamethicin-induced K+ efflux from Sf9 and Cf1 insect cells. Guihard, G., Falk, S., Vachon, V., Laprade, R., Schwartz, J.L. Biochemistry (1999) [Pubmed]
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  28. Purification, cloning, and bacterial expression of retinol dehydratase from Spodoptera frugiperda. Grün, F., Noy, N., Hämmerling, U., Buck, J. J. Biol. Chem. (1996) [Pubmed]
  29. Regulation of ecdysteroid signaling: cloning and characterization of ecdysone oxidase: a novel steroid oxidase from the cotton leafworm, Spodoptera littoralis. Takeuchi, H., Chen, J.H., O'Reilly, D.R., Turner, P.C., Rees, H.H. J. Biol. Chem. (2001) [Pubmed]
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