Disease relevance of Tospovirus

• The glycoprotein precursor (G1/G2) gene of tomato spotted wilt virus (TSWV) was expressed in BHK cells using the Semliki Forest virus expression system [1].
• An RNA-dependent RNA polymerase activity associated with virions of tomato spotted wilt virus, a plant- and insect-infecting bunyavirus [2].
• The tubule-forming NSm protein from Tomato spotted wilt virus complements cell-to-cell and long-distance movement of Tobacco mosaic virus hybrids [3].

High impact information on Tospovirus

• This peptide of 29 aa strongly interacts with the nucleocapsid proteins (N) of different tospoviruses [4].
• The nonstructural NSm protein of tomato spotted wilt tospovirus (TSWV) represents a putative viral movement protein involved in cell-to-cell movement of nonenveloped ribonucleocapsid structures [5].
• The movement protein NSm of tomato spotted wilt tospovirus (TSWV): RNA binding, interaction with the TSWV N protein, and identification of interacting plant proteins [5].
• We show that constitutive expression of the NSM viral movement protein (MP) of tomato spotted wilt virus in Nicotiana tabacum is sufficient to induce severe, infection-like symptoms, including pronounced deficiencies in root and shoot development [6].
• Expression and characterization of a soluble form of tomato spotted wilt virus glycoprotein GN [7].

Chemical compound and disease context of Tospovirus

• Effect of tiazofurin on tomato plants infected with tomato spotted wilt virus [8].
• Inhibitory effect of Distamycin-A and a pyrazino-pyrazine derivative on tomato spotted wilt virus [9].
• Effect of virazole (ribavirin) on tomato spotted wilt virus in two systemic hosts, tomato and tobacco [10].
• Distamycin-A hydrochloride, a synthetic antibiotic, and 2,3-dihydroxy-6-bromo-pyrazino (2,3-beta) pyrazine derivative, were used against tomato spotted wilt virus (TSWV) in tobacco plants [9].
• Actigard and Admire TD treatments significantly reduced the seasonal incidence of tomato spotted wilt virus (TSWV) symptomatic plants in 2 yr in the study [11].

Gene context of Tospovirus

• Tomato spotted wilt virus L RNA encodes a putative RNA polymerase [12].
• The present study demonstrates that the use of GST fusion proteins caused false positives during selection of phage-displayed single-chain antibody fragments (scFvs) specific for three domains of the movement protein (NS(M)) of tomato spotted wilt virus (TSWV) [13].
• One ORF (1 320 nucleotides in the viral sense strand) encodes a Mr 49.5 kDa protein, identified as the nonstructural (NSs) protein based on similarity to published tospovirus sequences [14].
• The L protein had highest identity in the 'core-polymerase domain' with the corresponding regions of other tospoviruses, tomato spotted wilt virus and impatiens necrotic spot virus [15].
• Homotypic interaction and multimerization of nucleocapsid protein of tomato spotted wilt tospovirus: identification and characterization of two interacting domains [16].

Analytical, diagnostic and therapeutic context of Tospovirus

• Multiple sequence alignment of the NSm and G1/G2 precursor proteins of IYSV with those of other tospoviruses, showed highest homologies to Peanut bud necrosis virus (PBNV) and Watermelon silver mottle virus (WSMV) [17].

References