Disease relevance of Orbivirus

• Eight viruses of the Corriparta serogroup (Reoviridae: Orbivirus) that were known to be heterogeneous on the basis of serology and polyacrylamide gel electrophoresis were examined by reciprocal RNA-RNA blot hybridization of genomic RNA [1].
• Methyltransferase activity of the insect orbivirus JKT-7400: photoaffinity labeling of a minor virion protein, VP4, with S-adenosylmethionine [2].
• Expression and antigenic characterization of the major core protein VP7 of Chuzan virus, a member of the Palyam serogroup orbiviruses [3].
• Pairs of oligodeoxynucleotide primers whose sequences were based on those of RNA segment 3 that encodes the bluetongue virus serogroup-reactive protein VP3, were synthesized for three BTVs from different geographic regions of the world and for seven Australian orbiviruses [4].
• Minor structural protein VP6 is the putative helicase of African horse sickness virus (AHSV), of the genus Orbivirus in the Reoviridae family [5].

High impact information on Orbivirus

• An orbivirus of mosquitoes which induces CO2 sensitivity in mosquitoes and is lethal for rabbits [6].
• Added proof of orbivirus replication was obtained through the use of tritiated uridine injections and the recovery of labeled virus in gut contents at the time of autopsy [7].
• Analysis of VP1 (Pol) and VP2 (T2, which correlates with the virus serogroup) indicated that YUOV is a new species of the genus Orbivirus that is unrelated to the other insect-borne orbiviruses [8].
• The NS3 gene and protein sequences of South African isolates of these viruses were determined, analysed and compared with cognate orbivirus genes from around the world [9].
• Co-expressed EEV VP3 and VP7 assembled into core-like particles that have typical orbivirus capsomeres [10].

Chemical compound and disease context of Orbivirus

• Although the dsRNA polyacrylamide gel profiles were unique for each isolate examined, the CTF genes did not exhibit sequence divergence as has been seen among other Orbivirus serogroups [11].
• Improved enzyme-linked immunosorbent assay for the detection of orbivirus antigens by treatment with sodium dodecyl sulfate [12].
• The Australian orbivirus serogrouping ELISA (SG-ELISA) utilised a sodium deoxycholate-treated cell lysate preparation from infected BHK cells which was subsequently probed in an indirect ELISA format with polyclonal antibodies representative of each serogroup [13].
• RNA of partially purified Kemerovo virus sedimented in a relatively broad region in sucrose density gradient with a peak at 14 S. Approximately 65% of this RNA was resistant to the action of ribonuclease which indicates the double-stranded nature of Kemerovo virus RNA [14].

Biological context of Orbivirus

• Polyacrylamide gel electrophoresis of the genomes of two orbiviruses: D'Aguilar and Eubenangee [15].

Gene context of Orbivirus

• The NS1 protein assembles into tubular structures, which are characteristically produced during orbivirus replication in infected cells [16].
• Various other comparative analyses of the proteins indicate that the VP2 species of the three orbiviruses are similar [17].
• Comparative sequence analysis with other serogroups of the genus Orbivirus indicated that the outer capsid protein VP2, which is the neutralizing antigen, appears to be the most variable and the major core protein VP3 is the most conserved [18].
• A comparison of the genes which encode non-structural protein NS3 of different orbiviruses [19].
• The structure and single-stranded (ss) RNA-binding by the nonstructural protein NS2 of three different orbiviruses were studied and compared [20].

Analytical, diagnostic and therapeutic context of Orbivirus

• Comparative sequence analysis with other serogroups of the genus Orbivirus suggested that VP2 is the principal determinant of serotype specificity and the neutralizing antigen of the Palyam serogroup [21].
• Immunoprecipitation of in vitro translation products directed by total BTV-17 RNA with a monoclonal antibody that reacts with all the serotypes of BTV and some other orbiviruses and precipitates VP7 directly demonstrated that gene segment 8 codes for this intergroup-specific polypeptide [22].
• Sedimentation analysis and nonreducing SDS-PAGE revealed that NS2 of all three orbiviruses is a 7S multimer with both inter- and intramolecular disulfide bonds, probably consisting of six or more NS2 molecules [20].
• However, the Mab against VP7 of AHSV cross-reacted with Chuzan virus by the indirect immunofluorescence assay (IFA), confirming the presence of conserved domain of VP7 among Orbiviruses [23].

References