High impact information on Genes, T-Cell Receptor alpha

• Taken together these results demonstrate that hGATA-3 is a novel lineage-specific hematopoietic transcription factor that appears to play an important role in regulating the T cell-specific expression of the TCR alpha gene [1].
• The calcium ionophore A23187 induces the accumulation of TCR-alpha but not -beta transcripts; and in contrast to CHX, it increases the rate of TCR-alpha gene transcription and the expression of large nuclear TCR-alpha precursor transcripts [2].
• The protein synthesis inhibitor cycloheximide (CHX) induces a 20-fold increase in mature TCR-alpha transcript accumulation without a concomitant increase in TCR-alpha gene transcription suggesting that CHX reverses the nuclear post-transcriptional events which prevent mature TCR-alpha mRNA accumulation [2].
• PFGE analysis of the T cell line Molt 4 suggests a greater than 600-kb deletion involving the TCR-alpha gene [3].
• The C delta 1 gene consists of two exons, one that encodes an extracellular Ig-like domain (exon 1) and the other that encodes either a very short or possibly a lacking CP region, a transmembrane region, and a cytoplasmic tail (exon 2); these are located within TCR alpha gene locus [4].

Anatomical context of Genes, T-Cell Receptor alpha

• To investigate this question, mixed radiation bone marrow chimeras were established in which wild-type T cell precursors capable of full maturation were diluted with precursors deficient in maturation potential because of targeted mutations of the RAG1 or TCR-alpha genes [5].

Gene context of Genes, T-Cell Receptor alpha

• These experiments suggest that cross-linking of the CD3 modules on pro-T cells mimics the signaling function expected of the pre-TcR complex, which is found at the surface of pre-T cells prior to functional TcR alpha gene rearrangement [6].
• There was a 10-fold increase in the 1.3-kb TcR beta transcript and a twofold increase in TcR alpha gene expression, even though Thy-1 expression was in general similar to controls [7].
• The functional rearranged TCR alpha gene is composed of a member of the V alpha 1 family, the junctional gene J alpha TT11 and C alpha [8].
• To further study this question, we have utilized a polymerase chain reaction-based strategy to isolate and sequence excision products of secondary TCR alpha gene rearrangement events [9].
• We conclude that there is a marked non random TCR alpha gene distribution in atopy using both V alpha family and anchored PCR [10].

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