Disease relevance of Melilotus

• The sweet clover differed from alfalfa in nodulation by a mutant strain of R. meliloti, but the agglutinins were indistinguishable by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Rhizobium agglutination, and cross-reactivity to antibodies [1].
• Vitamin K3 levels of 0, 0.45, 4.5, 11, and 45 mg/kg body weight/day were fed to 173-235-kg steers consuming toxic (40-50 ppm dicumarol) sweet clover [2].

High impact information on Melilotus

• Mutations in these genes affect nodulation of their natural plant hosts Medicago sativa and Melilotus albus to different extents and hsnD mutants have an altered host-range [3].
• In exoA, -F, and -H mutants, which are deficient in normal succinoglycan secretion and nodule invasion, a transposon Tn5 insertion in mucR or the presence of pMuc resulted in EPSb secretion and a restoration of nodule invasion on Medicago sativa and Melilotus alba [4].
• These experiments indicate that nodD1 and nodD3 have equivalent roles in nodulating sweet clover but that nodD1 plays a more important role than nodD3 in eliciting nodules on alfalfa [5].
• The nodD2 gene appears to have some effect on alfalfa nodulation and none on sweet clover [5].
• The expression of MaEXP1, a Melilotus alba expansin gene, is upregulated during the sweetclover-Sinorhizobium meliloti interaction [6].

Biological context of Melilotus

• R. meliloti strains containing mutations in the three nodD genes in all possible combinations were constructed and their nodulation phenotypes were assayed on Medicago sativa (alfalfa) and Melilotus alba (sweet clover) [5].
• On particular hosts, such as Medicago littoralis, mutants of the n5 operon showed a very weak nodule-forming ability, associated with a drastic decrease in the number of infection threads, while nodulation of Medicago truncatula or Melilotus alba was not affected [7].

Associations of Melilotus with chemical compounds

• Dicoumarol (moldy sweet clover) toxicosis in a group of Holstein calves [8].
• Comparing Se concentration between preplant and postharvest, there was a detectable reduction in the soil selenate, selenite, and water-extractable organic Se in the tall fescue and melilotus plots [9].
• Evaluation of vitamin K3 feed additive for prevention of sweet clover disease [2].
• A new oleanene glucuronide obtained from the aerial parts of Melilotus officinalis [10].
• In vitro availability of kaempferol glycosides from cream formulations of methanolic extract of the leaves of Melilotus elegans [11].

Gene context of Melilotus

• Proceedings: Studies on a cinnamic acid hydroxylase from chloroplasts of sweet clover, Melilotus alba desr [12].

References