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Parcerisa, J. et al.

Analysis of olive and hazelnut oil mixtures by high-performance liquid chromatography-atmospheric pressure chemical ionisation mass spectrometry of triacylglycerols and gas-liquid chromatography of non-saponifiable compounds (tocopherols and sterols).

We analysed the triacylglycerol, tocopherol and sterol composition of hazelnut oil, olive oil and their mixtures (90% olive oil with 10% hazelnut oil, 70% olive with 30% hazelnut oil and 50% olive oil with 50% hazelnut oil). The main triacylglycerols were 1,2,3-trioleylglycerol, 2,3-dioleyl-1-palmitoylglycerol, 2,3-dioleyl-1-linoleylglycerol and 2,3-dioleyl-1-stearoylglycerol. Non-saponfiable compounds (tocopherols and sterols) were derivatised as O-trimethylsilyl ethers. Alpha-tocopherol was the main vitamin E isomer in all samples; however, small amounts of beta-tocopherol and gamma-tocopherol were also found. Beta-sitosterol and delta5-avenasterol were the principal sterols in all samples; campesterol and stigmasterol were minor sterol compounds in all samples. Obtusifoliol, which was a major sterol in olive oil and oil mixtures, was not found in hazelnut oil. The discriminant analysis showed that hazelnut oil, olive oil and oil mixtures were clearly separated according to their triacylglycerol composition.[1]

References

Analysis of olive and hazelnut oil mixtures by high-performance liquid chromatography-atmospheric pressure chemical ionisation mass spectrometry of triacylglycerols and gas-liquid chromatography of non-saponifiable compounds (tocopherols and sterols). Parcerisa, J., Casals, I., Boatella, J., Codony, R., Rafecas, M. Journal of chromatography. A. (2000) [Pubmed]

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