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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Birth of a piglet derived from an oocyte fertilized by intracytoplasmic sperm injection (ICSI).

This report is of a live piglet derived from an embryo produced by intracytoplasmic sperm injection and carried to term. In vivo-matured oocytes were injected with in vitro-capacitated spermatozoa and after 40 h of culture, 2 to 4-cell-stage embryos were transferred to the oviducts of oestrus induced gilts 24 h after the expected ovulation time. Pregnancy of the surrogate mothers was maintained by estrogen treatment on days 10 to 16 after transfer. The single piglet that was born to a mother that had received 32 embryos was underweight, though healthy and lively.[1]

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