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Okamura, N. et al.

Okamura, Maki, Ishida, Uraguchi, Onishi, Sadasue, Tsuruta, Yagi,

Dissolution profiles of principal ingredients in Kampo medicinal powders by high-performance liquid chromatography.

We describe here a method using HPLC for the simultaneous determination of albiflorin, paeoniflorin, glycyrrhizin and six flavanone glycosides (liquiritin, liquiritin apioside, naringin, neohesperidin, hesperidin and narirutin) in the Kampo medicines, Shigyaku-san and Haino-san. All nine components were separated in less than 40 min by linear gradient elution using a mobile phase containing aqueous phosphoric acid and acetonitrile. The dissolution of these components from powders of Shigyaku-san in aqueous solution at pH 1.80, 4.08 and 6.89 was examined. All of the components except glycyrrhizin were dissolved entirely within 5 min regardless of pH. Dissolution of glycyrrhizin was dependent on the pH of the aqueous solution, and increased with increasing pH.[1]

References

Dissolution profiles of principal ingredients in Kampo medicinal powders by high-performance liquid chromatography. Okamura, N., Maki, T., Ishida, S., Uraguchi, C., Onishi, Y., Sadasue, E., Tsuruta, Y., Yagi, A. Chem. Pharm. Bull. (2000) [Pubmed]

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