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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

Membrane-facilitated bioproduction of 3-methylcatechol in an octanol/water two-phase system.

Bioproduction of 3-methylcatechol from toluene by Pseudomonas putida MC2 was studied in the presence of an additional 1-octanol phase. This solvent was used to supply the substrate and extract the product, in order to keep the aqueous concentrations low. A hollow-fibre membrane kept the octanol and aqueous phase separated to prevent phase toxicity towards the bacterium. Volumetric production rates increased approximately 40% as compared to two-phase 3-methylcatechol production with direct phase contact. Preliminary investigations on downstream processing of 3-methylcatechol showed that 1 M of sodium hydroxide selectively extracted the disodium salt of 3-methylcatechol into an aqueous phase.[1]

References

  1. Membrane-facilitated bioproduction of 3-methylcatechol in an octanol/water two-phase system. Hüsken, L.E., Oomes, M., Schroën, K., Tramper, J., de Bont, J.A., Beeftink, R. J. Biotechnol. (2002) [Pubmed]
 
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