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Kelley, T.F. et al.

Kelley, Sutton, Wallace, Wong,

Chondrocyte repopulation of allograft cartilage: a preliminary investigation and strategy for developing cartilage matrices for reconstruction.

OBJECTIVE: To evaluate the ability of devitalized cartilage to sustain repopulation by allogenic chondrocytes and to use 2-photon microscopy to assess cultured chondrocyte viability. STUDY DESIGN AND SETTING: At an academic medical center, we conducted experimental analysis of lagomorph nasal septa devitalized by subjecting them to either 1) chemical dehydration in ethanol, 2) lyophilization (freeze dry), or 3) repeated freeze-thaw (12 cycles) in liquid nitrogen. The processed cartilages were seeded with chondrocytes isolated from the septa of either the same rabbit or another rabbit and cultured for 4 weeks. They were assessed with a variety of techniques. RESULTS: The seeded chondrocytes maintained viability on the devitalized cartilage throughout the study period. Two-photon microscopy was effective in assessing the viability of cultured chondrocytes. CONCLUSION: Isolated lagomorph chondrocytes can be maintained in allogenic cartilage grafts devitalized using conventional physicochemical techniques. SIGNIFICANCE: This is the first experimental study to demonstrate that cultured chondrocytes can be maintained on devitalized cartilage.[1]

References

Chondrocyte repopulation of allograft cartilage: a preliminary investigation and strategy for developing cartilage matrices for reconstruction. Kelley, T.F., Sutton, F.M., Wallace, V.P., Wong, B.J. Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery. (2002) [Pubmed]

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