Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Theise, N.D. et al.

Theise, Henegariu, Grove, Jagirdar, Kao, Crawford, Badve, Saxena, Krause,

Radiation pneumonitis in mice: a severe injury model for pneumocyte engraftment from bone marrow.

OBJECTIVE: To better understand the process by which pneumocytes can be derived from bone marrow cells, we investigated the in vivo kinetics of such engraftment following lethal irradiation. METHODS: A cohort of lethally irradiated B6D2F1 female mice received whole bone marrow transplants (BMT) from age-matched male donors and were sacrificed at days 1, 3, 5, and 7 and months 2, 4, and 6 post-BMT (n = 3 for each time point). Additionally, 2 female mice who had received 200 male fluorescence-activated cell sorter (FACS)-sorted CD34(+)lin(-) cells were sacrificed 8 months post-BMT. RESULTS: Lethal irradiation caused histologic evidence of pneumonitis including alveolar breakdown and hemorrhage beginning at day 3. To identify male-derived pneumocytes, simultaneous fluorescence in situ hybridization (FISH) for Y-chromosome and surfactant B messenger RNA was performed on lung tissue. Y(+) type II pneumocytes were engrafted as early as day 5 posttransplant, and eventually from 2 to 14% of the pneumocytes were donor derived in individual mice. Co-staining for epithelial-specific cytokeratins demonstrated that by 2 months, marrow-derived pneumocytes could comprise entire alveoli, suggesting that type I cells derived from type II pneumocytes. CONCLUSIONS: We conclude that alveolar lining cells derive from bone marrow cells immediately after acute injury. Also, the CD34(+)lin(-) subpopulation is capable of such pulmonary engraftment.[1]

References

Radiation pneumonitis in mice: a severe injury model for pneumocyte engraftment from bone marrow. Theise, N.D., Henegariu, O., Grove, J., Jagirdar, J., Kao, P.N., Crawford, J.M., Badve, S., Saxena, R., Krause, D.S. Exp. Hematol. (2002) [Pubmed]

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