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Deaciuc, I.V. et al.

Deaciuc, Doherty, Burikhanov, Lee, Stromberg, Peng, de Villiers,

Large-scale gene profiling of the liver in a mouse model of chronic, intragastric ethanol infusion.

BACKGROUND/AIMS: The mechanisms underlying alcohol-induced liver injury are not fully elucidated. An approach in this direction would consist of an all-inclusive assessment of gene expression in the liver. The purpose of this study was to perform a comprehensive analysis of gene expression in the livers of mice treated with ethanol by means of intragastric infusion. METHODS: An ethanol- or glucose-enriched liquid diet was fed to animals for 4 weeks via a long-term gastrostomy catheter. The animals were killed and plasma alanine:2-oxoglutarate aminotransferase ( ALT) assay, liver histology and total RNA analysis by microarray gene technology were performed. RESULTS: Alcohol increased ALT, induced steatosis, necrosis and inflammation. A total of 12,423 genes were analyzed for expression out of which 4867 were expressed by the liver. Alcohol repressed expression of 11 genes, induced expression of 13 genes, and up- or down-regulated expression of 44 and 42 genes >2-fold, respectively. Gene expression analysis identified several genes that have not previously been tested for alcohol effects. CONCLUSIONS: This study: (i) expands the knowledge of mechanism(s) of action of ethanol; (ii) indicates novel pathways of ethanol action on the liver, and (iii) illustrates the utility of microarray gene analysis in hepatology research.[1]

References

Large-scale gene profiling of the liver in a mouse model of chronic, intragastric ethanol infusion. Deaciuc, I.V., Doherty, D.E., Burikhanov, R., Lee, E.Y., Stromberg, A.J., Peng, X., de Villiers, W.J. J. Hepatol. (2004) [Pubmed]

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