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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Dihydroorotate dehydrogenase mRNA and protein expression analysis in normal and drug-resistant cells.

To follow the expression of the fourth enzyme of pyrimidine de novo synthesis dihydroorotate dehydrogenase ( DHODH) in cells and tissues, we studied the DHODH mRNA expression by means of RT-PCR in rat tissues. Rabbit polyclonal anti- DHODH immunoglobulins were applied for immunochemical quantification of the enzyme protein by Western blotting. In mouse B-lymphocytes, which were adapted to tolerate up to a 50-fold concentration of the DHODH inhibitor leflunomide, a 20 fold protein overexpression was measured. Southern blotting indicated DHODH gene amplification.[1]

References

  1. Dihydroorotate dehydrogenase mRNA and protein expression analysis in normal and drug-resistant cells. Löffler, M., Klein, A., Hayek-Ouassini, M., Knecht, W., Konrad, L. Nucleosides Nucleotides Nucleic Acids (2004) [Pubmed]
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