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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Hanson, K.M. et al.

Rapid assessment of beta-asarone content of Acorus calamus by micellar electrokinetic capillary chromatography.

This report outlines a rapid, reproducible method for the determination of beta-asarone, a known carcinogen, using micellar electrokinetic capillary chromatography (MEKC)-UV-vis absorbance and a simple alcohol extraction. The MEKC method is based on a running buffer comprised of 100 mM sodium dodecyl sulfate (SDS), pH 10. The method is reproducible and provides baseline separation of alpha-asarone and beta-asarone. This protocol was used to determine the beta-asarone content of Acorus calamus rhizome of a diploid variety harvested from the wetlands of the United States and the triploid variety from India obtained commercially. The results indicate raw product that originated from India contained 4.4% w/w beta-asarone, while that from the United States contained 0.2% w/w beta-asarone. Neither sample contained detectable concentrations of alpha-asarone. This is the first report of the use of MEKC to determine asarone in a natural source.[1]

References

Rapid assessment of beta-asarone content of Acorus calamus by micellar electrokinetic capillary chromatography. Hanson, K.M., Gayton-Ely, M., Holland, L.A., Zehr, P.S., Söderberg, B.C. Electrophoresis (2005) [Pubmed]

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