Disease relevance of Azaron

• Mutagenicity testing of beta-asarone and commercial calamus drugs with Salmonella typhimurium [1].
• The growth of Bacillus subtilis, Erwinia carotovora subsp. carotovora, Ralstonia solanacearum, and Xanthomonas campestris pv. vesicatoria was slightly suppressed by beta-asarone [2].

High impact information on Azaron

• This protocol was used to determine the beta-asarone content of Acorus calamus rhizome of a diploid variety harvested from the wetlands of the United States and the triploid variety from India obtained commercially [3].
• Asarones (alpha-asarone and beta-asarone) are the active components in the traditional Chinese medicine (TCM) of Acorus tatarinowii Schott, which has been used to treat epilepsy for several thousand years [4].
• Determination of beta-asarone, safrole, isosafrole and anethole in alcoholic drinks by high-performance liquid chromatography [5].
• The main components of the SFE oil of A. calamus (Y = 3.5%) were acorenone, 13.4%; iso-acorone, 11.6%; (Z)-sesquilavandulol, 11.0%; dehydroxy isocalamendiol, 7.7%; and beta-asarone, 5.5% [6].
• Based on the receptor-ligand binding studies using a use-dependent NMDA receptor-channel blocker [3H]MK-801, asarone inhibited the specific bindings in a concentration-dependent fashion [7].

Biological context of Azaron

• The IC50 values for the alpha-, beta-, and the isolated asarone were 89.7, 121.7, and 279.5 microg/ml, respectively [7].

Anatomical context of Azaron

• Genotoxicity of the alkenylbenzenes alpha- and beta-asarone, myristicin and elimicin as determined by the UDS assay in cultured rat hepatocytes [8].
• A significant increase in the number of micronuclei with beta-asarone, coumarin, monocrotaline and retrorsine could be detected in the presence of S9-mix and the cell line Hep-G2 [9].

Associations of Azaron with other chemical compounds

• The isolated asarone inhibited the excitotoxicity induced by the exposure of cortical cultures for 15 min to 300 microM NMDA in a concentration-dependent manner, with the IC50 of 56.1 microg/ml [7].
• The method reported here, based on gas chromatography with selected-ion monitoring mass spectrometry (SIM), allows the simultaneous measurement of alpha, beta-thujone, pulegone, safrole, coumarin and beta-asarone at the ppb level in alcoholic beverages [10].

Analytical, diagnostic and therapeutic context of Azaron

• This is the first report of the use of MEKC to determine asarone in a natural source [3].
• Disposition of asarone after intravenous administration to rabbits assessed using HPLC [11].
• Scanning densitometry with absorption measurement at 313 nm allows specific, accurate, and precise quantification of beta-asarone [12].
• Quantitative determination of beta-asarone in calamus by high-performance thin-layer chromatography [12].

References