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Lambert, A.J. et al.

Lambert, Nasci, Cropp, Martin, Rose, Russell, Lanciotti,

Nucleic acid amplification assays for detection of La Crosse virus RNA.

We report the development of nucleic acid sequence-based amplification (NASBA) and quantitative real-time reverse transcription (RT)-PCR assays for the detection of La Crosse ( LAC) virus in field-collected vector mosquito samples and human clinical samples. The sensitivities of these assays were compared to that of a standard plaque assay in Vero cells. The NASBA and quantitative real-time RT-PCR assays demonstrated sensitivities greater than that of the standard plaque assay. The specificities of these assays were determined by testing a battery of reference strain viruses, including representative strains of LAC virus and other arthropod-borne viruses. Additionally, these assays were used to detect LAC viral RNA in mosquito pool samples and human brain tissue samples and yielded results within less than 4 h. The NASBA and quantitative real-time RT-PCR assays detect LAC viral RNA in a sensitive, specific, and rapid manner; these findings support the use of these assays in surveillance and diagnostic laboratory systems.[1]

References

Nucleic acid amplification assays for detection of La Crosse virus RNA. Lambert, A.J., Nasci, R.S., Cropp, B.C., Martin, D.A., Rose, B.C., Russell, B.J., Lanciotti, R.S. J. Clin. Microbiol. (2005) [Pubmed]

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