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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
MeSH Review

Self-Sustained Sequence Replication

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Disease relevance of Self-Sustained Sequence Replication


High impact information on Self-Sustained Sequence Replication


Chemical compound and disease context of Self-Sustained Sequence Replication

  • We thus recommend, for quantifying HIV-1 RNA by NASBA, to use preferably EDTA blood which is kept at room temperature until plasma separation [11].

Biological context of Self-Sustained Sequence Replication

  • Surprisingly, we found that the nasBA operon is tightly linked to the narL-narGHJI region in K. pneumoniae, even though the nitrate assimilatory and respiratory enzymes serve different physiological functions [12].
  • We report the development of nucleic acid sequence-based amplification (NASBA) and quantitative real-time reverse transcription (RT)-PCR assays for the detection of La Crosse (LAC) virus in field-collected vector mosquito samples and human clinical samples [13].
  • Thus, NASBA is a sensitive and specific means of rapidly determining CCR5 genotype and provides several technical advantages over alternative assay systems [14].
  • These findings suggest that the quantification of WT1 gene expression by competitive NASBA is useful in assessing therapeutic effects and detecting MRD [15].

Anatomical context of Self-Sustained Sequence Replication

  • The purpose of this study was to determine MDR1 mRNA expression levels in peripheral blood leukocytes obtained from healthy adult volunteers using a competitive nucleic acid sequence-based amplification (NASBA) assay [16].
  • A specific NASBA reaction was developed for the detection of vIL-10 and BDLF2 transcripts and this was tested initially on cell lines and later on clinical samples [17].
  • These results demonstrate that real-time NASBA provides a rapid and sensitive alternative to RT-PCR for the routine qualitative assay of sputum for SARS corona viral RNA detection [18].

Associations of Self-Sustained Sequence Replication with chemical compounds

  • The Basic Kit provides an open platform for RNA amplification and detection and contains isolation reagents for nucleic acid extraction, nucleic acid sequence-based amplification (NASBA) reagents (enzymes and buffers), and a generic ruthenium-labeled probe for electrochemiluminescent (ECL) detection of amplified product [19].
  • Of the 67 samples found positive by the C6/36 assay, 66 were found positive by the NASBA assay, for a sensitivity of 98.5% [20].
  • The conditions for NASBA amplification of the16S rRNA target were optimized (90 mM KCl, 12 mM MgCl(2), 0.2 microM P1 and P2 primers), amplified RNA was captured by a biotin-labelled capture probe immobilized onto streptavidin coated microtiter plates and detected with an FITC-labelled oligonucleotide probe and Aequorin-anti-FITC antibody conjugate [4].
  • Of the single-step RNA purification methods, good results were obtained with the TRIzol method (Gibco Life Technologies, Paisley, UK) and with the extraction method offered by the NASBA kit (Organon Teknika, Turnhout, Belgium) [21].
  • A quantitative nucleic acid sequence-based amplification (QT-NASBA) assay was employed to predict retrospectively the outcome of sulfadoxine-pyrimethamine (SP) treatment of uncomplicated malaria in children aged <6 years in an endemic region [22].

Gene context of Self-Sustained Sequence Replication

  • For further evaluation, detection of E6 and E7 mRNA from HPV types 35, 52, and 58 by real-time multiplex nucleic acid sequence-based amplification was also included [23].
  • We developed a qualitative CCR5 genotyping assay using NASBA, an isothermal nucleic acid amplification technology [14].
  • A nested set of four primers flanking the BCR-ABL junction was used in two serial NASBA reactions performed for 2 hours [24].
  • Among the 45 seropositives, 34 of which were tested for p24 antigen, 43 (96%) were positive by NASBA and 41 (91%) by RNA-PCR [25].
  • RT-PCRs specific for the different splice variants of the BARTs and both a nucleic acid sequence-based amplification assay and an RT-PCR specific for the BARF0 ORF were used [26].

Analytical, diagnostic and therapeutic context of Self-Sustained Sequence Replication

  • Therefore, NASBA is unique for sensitive detection of transcription of intronless genes, which preclude strategies such as intron spanning primer pairs to control false positive results in RT-PCR [27].
  • METHODS: Overall, 82 heart (HTR) and lung (LTR) transplant recipients were randomized into two arms, where therapy was guided by qualitative pp67 mRNA NASBA (40 patients) or quantitative antigenemia (42 patients) [28].
  • The sensitivity of the NASBA assay was comparable to that of a PCR targeted to the P1 adhesin gene [29].
  • METHODS: The results of pp67-mRNA NASBA, DNA-PCR, culture and pp65-antigenemia assay were compared in 402 whole blood specimens of 98 HIV-infected patients with a low CD4 lymphocyte count who had not yet received highly active antiretroviral therapy (HAART) [30].
  • Oligonucleotide primers targeting gene 9 encoding a serotype-specific antigen VP7 were selected and used for the amplification of viral RNA by the isothermal NASBA process, resulting in the accumulation of biotinylated RNA amplicons [31].


  1. Study on mutations and antiretroviral therapy (SMART): preliminary results. Gianotti, N., Moretti, F., Tambussi, G., Racca, S., Presi, S., Crucianelli, R., Carrera, P., Ferrari, M., Lazzarin, A. Antivir. Ther. (Lond.) (1999) [Pubmed]
  2. Unique transcription pattern of Epstein-Barr virus (EBV) in EBV-carrying gastric adenocarcinomas: expression of the transforming BARF1 gene. zur Hausen, A., Brink, A.A., Craanen, M.E., Middeldorp, J.M., Meijer, C.J., van den Brule, A.J. Cancer Res. (2000) [Pubmed]
  3. Direct quantification of human cytomegalovirus immediate-early and late mRNA levels in blood of lung transplant recipients by competitive nucleic acid sequence-based amplification. Greijer, A.E., Verschuuren, E.A., Harmsen, M.C., Dekkers, C.A., Adriaanse, H.M., The, T.H., Middeldorp, J.M. J. Clin. Microbiol. (2001) [Pubmed]
  4. Quantitation of Chlamydia trachomatis 16S rRNA using NASBA amplification and a bioluminescent microtiter plate assay. Song, X., Coombes, B.K., Mahony, J.B. Comb. Chem. High Throughput Screen. (2000) [Pubmed]
  5. Rapid and sensitive detection of avian influenza virus subtype H7 using NASBA. Collins, R.A., Ko, L.S., Fung, K.Y., Chan, K.Y., Xing, J., Lau, L.T., Yu, A.C. Biochem. Biophys. Res. Commun. (2003) [Pubmed]
  6. The in vivo kinetics of tissue factor messenger RNA expression during human endotoxemia: relationship with activation of coagulation. Franco, R.F., de Jonge, E., Dekkers, P.E., Timmerman, J.J., Spek, C.A., van Deventer, S.J., van Deursen, P., van Kerkhoff, L., van Gemen, B., ten Cate, H., van der Poll, T., Reitsma, P.H. Blood (2000) [Pubmed]
  7. Comparative evaluation of hepatitis C virus RNA quantitation by branched DNA, NASBA, and monitor assays. Lunel, F., Cresta, P., Vitour, D., Payan, C., Dumont, B., Frangeul, L., Reboul, D., Brault, C., Piette, J.C., Huraux, J.M. Hepatology (1999) [Pubmed]
  8. The effects of antiretroviral therapy on HIV-1 RNA loads in seminal plasma in HIV-positive patients with and without urethritis. Sadiq, S.T., Taylor, S., Kaye, S., Bennett, J., Johnstone, R., Byrne, P., Copas, A.J., Drake, S.M., Pillay, D., Weller, I. AIDS (2002) [Pubmed]
  9. Strong correlation between the complement-mediated antibody-dependent enhancement of HIV-1 infection and plasma viral load. Szabó, J., Prohászka, Z., Tóth, F.D., Gyuris, A., Segesdi, J., Bánhegyi, D., Ujhelyi, E., Minárovits, J., Füst, G. AIDS (1999) [Pubmed]
  10. Establishment of new transmissible and drug-sensitive human immunodeficiency virus type 1 wild types due to transmission of nucleoside analogue-resistant virus. de Ronde, A., van Dooren, M., van Der Hoek, L., Bouwhuis, D., de Rooij, E., van Gemen, B., de Boer, R., Goudsmit, J. J. Virol. (2001) [Pubmed]
  11. Stability of HIV-1 RNA in blood during specimen handling and storage prior to amplification by NASBA-QT. Bruisten, S.M., Oudshoorn, P., van Swieten, P., Boeser-Nunnink, B., van Aarle, P., Tondreau, S.P., Cuypers, H.T. J. Virol. Methods (1997) [Pubmed]
  12. Structures of genes nasA and nasB, encoding assimilatory nitrate and nitrite reductases in Klebsiella pneumoniae M5al. Lin, J.T., Goldman, B.S., Stewart, V. J. Bacteriol. (1993) [Pubmed]
  13. Nucleic acid amplification assays for detection of La Crosse virus RNA. Lambert, A.J., Nasci, R.S., Cropp, B.C., Martin, D.A., Rose, B.C., Russell, B.J., Lanciotti, R.S. J. Clin. Microbiol. (2005) [Pubmed]
  14. Genotyping of the CCR5 chemokine receptor by isothermal NASBA amplification and differential probe hybridization. Romano, J.W., Tetali, S., Lee, E.M., Shurtliff, R.N., Wang, X.P., Pahwa, S., Kaplan, M.H., Ginocchio, C.C. Clin. Diagn. Lab. Immunol. (1999) [Pubmed]
  15. Quantification of WT1 mRNA by competitive NASBA in AML patients. Fukahori, S. The Kurume medical journal. (2001) [Pubmed]
  16. Expression level of MDR1 message in peripheral blood leukocytes from healthy adults: a competitive nucleic acid sequence-based amplification assay for its determination. Kobayashi, H., Takemura, Y., Hayashi, T., Ujiiye, T., Kawase, M., Niino, Y., Miyachi, H., Ohshima, T., Hotta, T. Clin. Chem. Lab. Med. (2004) [Pubmed]
  17. Expression of Epstein-Barr virus (EBV) transcripts encoding homologues to important human proteins in diverse EBV associated diseases. Hayes, D.P., Brink, A.A., Vervoort, M.B., Middeldorp, J.M., Meijer, C.J., van den Brule, A.J. MP, Mol. Pathol. (1999) [Pubmed]
  18. Development and comparison of the real-time amplification based methods--NASBA-Beacon, RT-PCR taqman and RT-PCR hybridization probe assays--for the qualitative detection of sars coronavirus. Chantratita, W., Pongtanapisit, W., Piroj, W., Srichunrasmi, C., Seesuai, S. Southeast Asian J. Trop. Med. Public Health (2004) [Pubmed]
  19. Evaluation of the NucliSens Basic Kit for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in genital tract specimens using nucleic acid sequence-based amplification of 16S rRNA. Mahony, J.B., Song, X., Chong, S., Faught, M., Salonga, T., Kapala, J. J. Clin. Microbiol. (2001) [Pubmed]
  20. Detection of dengue viral RNA using a nucleic acid sequence-based amplification assay. Wu, S.J., Lee, E.M., Putvatana, R., Shurtliff, R.N., Porter, K.R., Suharyono, W., Watts, D.M., King, C.C., Murphy, G.S., Hayes, C.G., Romano, J.W. J. Clin. Microbiol. (2001) [Pubmed]
  21. Isolation of HIV-1 RNA from plasma: evaluation of eight different extraction methods. Verhofstede, C., Fransen, K., Marissens, D., Verhelst, R., van der Groen, G., Lauwers, S., Zissis, G., Plum, J. J. Virol. Methods (1996) [Pubmed]
  22. Plasmodium falciparum: evaluation of a quantitative nucleic acid sequence-based amplification assay to predict the outcome of sulfadoxine-pyrimethamine treatment of uncomplicated malaria. Omar, S.A., Mens, P.F., Schoone, G.J., Yusuf, A., Mwangi, J., Kaniaru, S., Omer, G.A., Schallig, H.D. Exp. Parasitol. (2005) [Pubmed]
  23. Presence of E6 and E7 mRNA from human papillomavirus types 16, 18, 31, 33, and 45 in the majority of cervical carcinomas. Kraus, I., Molden, T., Holm, R., Lie, A.K., Karlsen, F., Kristensen, G.B., Skomedal, H. J. Clin. Microbiol. (2006) [Pubmed]
  24. Detection and direct sequence identification of BCR-ABL mRNA in Ph+ chronic myeloid leukemia. Sooknanan, R., Malek, L., Wang, X.H., Siebert, T., Keating, A. Exp. Hematol. (1993) [Pubmed]
  25. Detection of HIV-1 RNA in plasma and serum samples using the NASBA amplification system compared to RNA-PCR. Vandamme, A.M., Van Dooren, S., Kok, W., Goubau, P., Fransen, K., Kievits, T., Schmit, J.C., De Clercq, E., Desmyter, J. J. Virol. Methods (1995) [Pubmed]
  26. In vivo transcription of the Epstein-Barr virus (EBV) BamHI-A region without associated in vivo BARF0 protein expression in multiple EBV-associated disorders. van Beek, J., Brink, A.A., Vervoort, M.B., van Zijp, M.J., Meijer, C.J., van den Brule, A.J., Middeldorp, J.M. J. Gen. Virol. (2003) [Pubmed]
  27. Highly sensitive detection of gene expression of an intronless gene: amplification of mRNA, but not genomic DNA by nucleic acid sequence based amplification (NASBA). Heim, A., Grumbach, I.M., Zeuke, S., Top, B. Nucleic Acids Res. (1998) [Pubmed]
  28. Human cytomegalovirus pp67 mRNAemia versus pp65 antigenemia for guiding preemptive therapy in heart and lung transplant recipients: a prospective, randomized, controlled, open-label trial. Gerna, G., Baldanti, F., Lilleri, D., Parea, M., Torsellini, M., Castiglioni, B., Vitulo, P., Pellegrini, C., Viganò, M., Grossi, P., Revello, M.G. Transplantation (2003) [Pubmed]
  29. Detection of Mycoplasma pneumoniae in spiked clinical samples by nucleic acid sequence-based amplification. Loens, K., Ursi, D., Ieven, M., van Aarle, P., Sillekens, P., Oudshoorn, P., Goossens, H. J. Clin. Microbiol. (2002) [Pubmed]
  30. Detection of late pp67-mRNA by NASBA in peripheral blood for the diagnosis of human cytomegalovirus disease in AIDS patients. Blank, B.S., Meenhorst, P.L., Pauw, W., Mulder, J.W., van Dijk, W.C., Smits, P.H., Roeles, F., Middeldorp, J.M., Lange, J.M. J. Clin. Virol. (2002) [Pubmed]
  31. Rapid detection of human rotavirus using colorimetric nucleic acid sequence-based amplification (NASBA)-enzyme-linked immunosorbent assay in sewage treatment effluent. Jean, J., Blais, B., Darveau, A., Fliss, I. FEMS Microbiol. Lett. (2002) [Pubmed]
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