Pectinesterase Inhibitor from Jelly Fig (Ficus awkeotsang Makino) Achene Induces Apoptosis of Human Leukemic U937 Cells.
The antitumor activity of pectinesterase inhibitor (PEI), a group of cationic polypeptides, from jelly fig (Ficus awkeotsang Makino) achene was first examined as a treatment for leukemia in this study. PEI displayed strong growth inhibition against human leukemic U937 cells via induction of apoptosis in a dose- and time-dependent manner. At a level of 50 mug/mL, PEI inhibited 90% of cell growth, and the concentration of PEI required to induce 50% of cell viability (LC(50)) was about 180 mug/mL. Meanwhile, cell cycle arrest at G2/M phase was observed when cells were incubated with 100 mug PEI/mL for 24 h. PEI displayed a dose-dependent influence on mitochondria transmembrane potential ( MTP, DeltaPsim) of cells when detected by a flow cytometry. MTP of more than 50% cells was reduced when cells were incubated with PEI at levels higher than 50 mug PEI/mL for 24 h. In addition, PEI upregulated caspase-3 activity. Taken together, PEI potently inhibited the proliferation of human leukemic U937 cells via cell cycle arrest and apoptosis in association with MTP reduction and caspase-3 activation, respectively, and showed therapy potential for U937 cells.[1]References
- Pectinesterase Inhibitor from Jelly Fig (Ficus awkeotsang Makino) Achene Induces Apoptosis of Human Leukemic U937 Cells. Chang, J.H., Wang, Y.T., Chang, H.M. Ann. N. Y. Acad. Sci. (2005) [Pubmed]
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