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MeSH Review

U937 Cells

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Disease relevance of U937 Cells

  • HIV-1 preparations from H9 and U937 cells, which express low levels of CD55 and CD59, had intermediate to high sensitivity while other cell line-derived viruses and primary isolates of HIV-1 were resistant to complement-mediated virolysis [1].
  • The effects of HIV infection on U937 cells strikingly resemble the effects of differentiation-inducing agents, such as PMA and DMSO, on the U937 phenotype [2].
  • In this study, we report that tat-defective HIV-1 and HIV-2 viral DNA transfected into U937 cells can direct efficient viral replication in the presence of transcriptional stimulators such as TNF-alpha and PMA [3].
  • These included inhibition of ICAM-1-dependent homotypic aggregation of Raji Burkitt lymphoma and phorbol-ester treated U937 cells at concentrations as low as 80 micrograms/ml (24 microM) [4].
  • Analysis of beta2 integrin mRNA and protein in U937 cells revealed a 5- to 6-fold increase with hypoxia [5].

High impact information on U937 Cells

  • Anti-CD15 antibodies inhibited the binding of U937 cells to PADGEM-expressing COS cells and to purified PADGEM incorporated into phospholipid vesicles [6].
  • Conditional expression of RUNX1-CBF2T1 in U937 cells downregulated CEBPA mRNA, protein and DNA binding activity [7].
  • NF-kappa B-binding activity appears during the transition from promonocyte to monocyte in U937 cells induced to differentiate in vitro and is present constitutively in mature monocytes and macrophages [8].
  • A subline of U937 cells (U937D) was obtained in which creatine kinase B (CK-B) messenger RNA was present and bound to ribosomes, but CK activity was undetectable [9].
  • The cDNA sequence of VPF from human U937 cells was shown to code for a 189-amino acid polypeptide that is similar in structure to the B chain of platelet-derived growth factor (PDGF-B) and other PDGF-B-related proteins [10].

Chemical compound and disease context of U937 Cells


Biological context of U937 Cells


Anatomical context of U937 Cells


Associations of U937 Cells with chemical compounds

  • This cDNA was used to clone cDNAs for human homologues of MIP-2 from a library prepared from phorbol myristate acetate-treated and LPS-stimulated U937 cells [26].
  • Experiments were performed using [14C-methyl]choline-labeled U937 cells and monocytes to determine whether IL-4R activated phospholipase C (PLC), PLD, or PLA2 to use membrane phosphatidylcholine (PC) to form DAG [27].
  • By immunoblot analysis, U937 cell glucan-binding proteins from detergent lysates contained two cell proteins antigenic for the anti-Id that were indistinguishable from affinity-purified molecules in size and subunit composition [28].
  • To characterize their structure, binding studies were carried out with human U937 cells and a rabbit IgG anti-Id that recognizes epitopes on monocyte beta-glucan receptors [28].
  • In summary, IL-4R signaling in monocytes and U937 cells involves PLC and not PLD, PLA2, or SMase, and it uses PC and not PIP2 to form DAG [27].

Gene context of U937 Cells

  • Ca2(+)-sensitive binding of thrombospondin to U937 cells is due to the formation of calcium precipitate in the binding medium [29].
  • Overexpression of PKC-zeta in G2 and U937 cells led to increased directional motility to SDF-1 [30].
  • In U937 cells large amounts of IL-1 beta are made but none is secreted [31].
  • Here, we report a novel cytoplasmic localization of p21(Cip1/WAF1) in peripheral blood monocytes (PBMs) and in U937 cells undergoing monocytic differentiation by in vitro treatment with vitamin D3 or ectopic expression of p21(Cip1/WAF1), and analyze the biological consequences of this cytoplasmic expression [32].
  • In conclusion, in U937 cells the binding to the receptor does not shield uPA from the action of PAI-1 [33].

Analytical, diagnostic and therapeutic context of U937 Cells


  1. Role of virion-associated glycosylphosphatidylinositol-linked proteins CD55 and CD59 in complement resistance of cell line-derived and primary isolates of HIV-1. Saifuddin, M., Parker, C.J., Peeples, M.E., Gorny, M.K., Zolla-Pazner, S., Ghassemi, M., Rooney, I.A., Atkinson, J.P., Spear, G.T. J. Exp. Med. (1995) [Pubmed]
  2. Human immunodeficiency virus infection down-regulates HLA class II expression and induces differentiation in promonocytic U937 cells. Petit, A.J., Terpstra, F.G., Miedema, F. J. Clin. Invest. (1987) [Pubmed]
  3. Tat-independent replication of human immunodeficiency viruses. Luznik, L., Kraus, G., Guatelli, J., Richman, D., Wong-Staal, F. J. Clin. Invest. (1995) [Pubmed]
  4. Inhibition of intercellular adhesion molecule 1-dependent biological activities by a synthetic peptide analog. Fecondo, J.V., Kent, S.B., Boyd, A.W. Proc. Natl. Acad. Sci. U.S.A. (1991) [Pubmed]
  5. Leukocyte adhesion during hypoxia is mediated by HIF-1-dependent induction of beta2 integrin gene expression. Kong, T., Eltzschig, H.K., Karhausen, J., Colgan, S.P., Shelley, C.S. Proc. Natl. Acad. Sci. U.S.A. (2004) [Pubmed]
  6. PADGEM-dependent adhesion of platelets to monocytes and neutrophils is mediated by a lineage-specific carbohydrate, LNF III (CD15). Larsen, E., Palabrica, T., Sajer, S., Gilbert, G.E., Wagner, D.D., Furie, B.C., Furie, B. Cell (1990) [Pubmed]
  7. AML1-ETO downregulates the granulocytic differentiation factor C/EBPalpha in t(8;21) myeloid leukemia. Pabst, T., Mueller, B.U., Harakawa, N., Schoch, C., Haferlach, T., Behre, G., Hiddemann, W., Zhang, D.E., Tenen, D.G. Nat. Med. (2001) [Pubmed]
  8. Activation of HIV gene expression during monocyte differentiation by induction of NF-kappa B. Griffin, G.E., Leung, K., Folks, T.M., Kunkel, S., Nabel, G.J. Nature (1989) [Pubmed]
  9. Reversal of creatine kinase translational repression by 3' untranslated sequences. Ch'ng, J.L., Shoemaker, D.L., Schimmel, P., Holmes, E.W. Science (1990) [Pubmed]
  10. Vascular permeability factor, an endothelial cell mitogen related to PDGF. Keck, P.J., Hauser, S.D., Krivi, G., Sanzo, K., Warren, T., Feder, J., Connolly, D.T. Science (1989) [Pubmed]
  11. Anticoagulant effects of retinoic acids on leukemia cells. Saito, T., Koyama, T., Nagata, K., Kamiyama, R., Hirosawa, S. Blood (1996) [Pubmed]
  12. Serum factor from patients with cirrhosis and hepatocellular carcinoma enhances production of prostaglandin E2 by U937 cells. Iwamoto, H., Sakamoto, S., Nawata, H. Hepatology (1991) [Pubmed]
  13. Role of sulfhydryl groups in induction of cell surface down-modulation and shedding of extracellular domain of human TNF receptors in human histiocytic lymphoma U937 cells. Zhang, L., Aggarwal, B.B. J. Immunol. (1994) [Pubmed]
  14. Apoptosis induced by cadmium in human lymphoma U937 cells through Ca2+-calpain and caspase-mitochondria- dependent pathways. Li, M., Kondo, T., Zhao, Q.L., Li, F.J., Tanabe, K., Arai, Y., Zhou, Z.C., Kasuya, M. J. Biol. Chem. (2000) [Pubmed]
  15. Increased human immunodeficiency virus (HIV) expression in chronically infected U937 cells upon in vitro differentiation by hydroxyvitamin D3: roles of interferon and tumor necrosis factor in regulation of HIV production. Locardi, C., Petrini, C., Boccoli, G., Testa, U., Dieffenbach, C., Buttò, S., Belardelli, F. J. Virol. (1990) [Pubmed]
  16. Cytokine-induced expression of HIV-1 in a chronically infected promonocyte cell line. Folks, T.M., Justement, J., Kinter, A., Dinarello, C.A., Fauci, A.S. Science (1987) [Pubmed]
  17. BLIMP-1: trigger for differentiation of myeloid lineage. Chang, D.H., Angelin-Duclos, C., Calame, K. Nat. Immunol. (2000) [Pubmed]
  18. Genetic analysis of the human tumor necrosis factor alpha/cachectin promoter region in a macrophage cell line. Economou, J.S., Rhoades, K., Essner, R., McBride, W.H., Gasson, J.C., Morton, D.L. J. Exp. Med. (1989) [Pubmed]
  19. Apoptotic signaling through CD95 (Fas/Apo-1) activates an acidic sphingomyelinase. Cifone, M.G., De Maria, R., Roncaioli, P., Rippo, M.R., Azuma, M., Lanier, L.L., Santoni, A., Testi, R. J. Exp. Med. (1994) [Pubmed]
  20. The PML/RARalpha fusion protein inhibits tumor necrosis factor-alpha-induced apoptosis in U937 cells and acute promyelocytic leukemia blasts. Testa, U., Grignani, F., Samoggia, P., Zanetti, C., Riccioni, R., Lo Coco, F., Diverio, D., Felli, N., Passerini, C.G., Grell, M., Pelicci, P.G., Peschle, C. J. Clin. Invest. (1998) [Pubmed]
  21. PADGEM protein: a receptor that mediates the interaction of activated platelets with neutrophils and monocytes. Larsen, E., Celi, A., Gilbert, G.E., Furie, B.C., Erban, J.K., Bonfanti, R., Wagner, D.D., Furie, B. Cell (1989) [Pubmed]
  22. Human eosinophils express functional interleukin 2 receptors. Rand, T.H., Silberstein, D.S., Kornfeld, H., Weller, P.F. J. Clin. Invest. (1991) [Pubmed]
  23. Human granulocyte-macrophage colony-stimulating factor induces expression of the tumor necrosis factor gene by the U937 cell line and by normal human monocytes. Cannistra, S.A., Rambaldi, A., Spriggs, D.R., Herrmann, F., Kufe, D., Griffin, J.D. J. Clin. Invest. (1987) [Pubmed]
  24. Activation-dependent recognition by hematopoietic cells of the LDV sequence in the V region of fibronectin. Wayner, E.A., Kovach, N.L. J. Cell Biol. (1992) [Pubmed]
  25. Purification of a 110-kilodalton cytosolic phospholipase A2 from the human monocytic cell line U937. Clark, J.D., Milona, N., Knopf, J.L. Proc. Natl. Acad. Sci. U.S.A. (1990) [Pubmed]
  26. Cloning and characterization of cDNAs for murine macrophage inflammatory protein 2 and its human homologues. Tekamp-Olson, P., Gallegos, C., Bauer, D., McClain, J., Sherry, B., Fabre, M., van Deventer, S., Cerami, A. J. Exp. Med. (1990) [Pubmed]
  27. Interleukin 4 receptor signaling in human monocytes and U937 cells involves the activation of a phosphatidylcholine-specific phospholipase C: a comparison with chemotactic peptide, FMLP, phospholipase D, and sphingomyelinase. Ho, J.L., Zhu, B., He, S., Du, B., Rothman, R. J. Exp. Med. (1994) [Pubmed]
  28. Isolation and characterization of beta-glucan receptors on human mononuclear phagocytes. Czop, J.K., Kay, J. J. Exp. Med. (1991) [Pubmed]
  29. Ca2(+)-sensitive binding of thrombospondin to U937 cells is due to the formation of calcium precipitate in the binding medium. Sun, X., Mosher, D.F. J. Clin. Invest. (1991) [Pubmed]
  30. Atypical PKC-zeta regulates SDF-1-mediated migration and development of human CD34+ progenitor cells. Petit, I., Goichberg, P., Spiegel, A., Peled, A., Brodie, C., Seger, R., Nagler, A., Alon, R., Lapidot, T. J. Clin. Invest. (2005) [Pubmed]
  31. A novel secretory pathway for interleukin-1 beta, a protein lacking a signal sequence. Rubartelli, A., Cozzolino, F., Talio, M., Sitia, R. EMBO J. (1990) [Pubmed]
  32. Apoptosis inhibitory activity of cytoplasmic p21(Cip1/WAF1) in monocytic differentiation. Asada, M., Yamada, T., Ichijo, H., Delia, D., Miyazono, K., Fukumuro, K., Mizutani, S. EMBO J. (1999) [Pubmed]
  33. Accessibility of receptor-bound urokinase to type-1 plasminogen activator inhibitor. Cubellis, M.V., Andreasen, P., Ragno, P., Mayer, M., Danø, K., Blasi, F. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  34. 12-o-Tetradecanoyl-phorbol-13-acetate-differentiated U937 cells express a macrophage-like profile of neutral proteinases. High levels of secreted collagenase and collagenase inhibitor accompany low levels of intracellular elastase and cathepsin G. Welgus, H.G., Connolly, N.L., Senior, R.M. J. Clin. Invest. (1986) [Pubmed]
  35. Regulation of urokinase receptors in monocytelike U937 cells by phorbol ester phorbol myristate acetate. Picone, R., Kajtaniak, E.L., Nielsen, L.S., Behrendt, N., Mastronicola, M.R., Cubellis, M.V., Stoppelli, M.P., Pedersen, S., Danø, K., Blasi, F. J. Cell Biol. (1989) [Pubmed]
  36. Proteolytic activation of PKN by caspase-3 or related protease during apoptosis. Takahashi, M., Mukai, H., Toshimori, M., Miyamoto, M., Ono, Y. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
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