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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Selective expression of cholesterol metabolism genes in normal CD34(+)CD38(-) cells with a heterogeneous expression pattern in AML cells.

OBJECTIVE: To identify adenosine triphosphate-binding-cassette (ABC) transporters that are selectively expressed in normal and/or leukemic CD34(+)CD38(-) stem cells. METHODS: Microarray experiments on murine stem cells identified 13 ABC transporters with a high expression level. Corresponding human transporters were analyzed in normal CD34(+)CD38(-) and CD34(+)CD38(+) bone marrow cells by quantitative reverse transcriptase polymerase chain reaction. RESULTS: Five ABC transporters, including ABCG1, were differentially expressed with a higher expression in CD34(+)CD38(-) cells. Besides ABCG1, known to be involved in cholesterol metabolism, expression of another major cholesterol transporter (ABCA1), some cholesterol metabolism genes (3-hydroxy-3-methyl-glutaryl-CoA reductase, low-density lipoprotein receptor), and the transcription factor controlling ABCA1 and ABCG1 expression, liver-X-receptor-alpha (LXR-alpha), were assessed. All these genes were predominantly expressed in the more primitive subpopulation, indicating a high rate of cholesterol metabolism and transport. Conversely in acute myeloid leukemia (AML), a heterogeneous expression pattern was found consisting of a considerably higher expression of particularly LXR-alpha in CD34(+) cells and a reverse expression pattern in a subset of AML CD34(+)CD38(+) cells. CONCLUSION: These data suggest an active cholesterol metabolism and efflux in normal CD34(+)CD38(-) cells, although a subgroup of AMLs potentially demonstrate a hyperactive cholesterol metabolism.[1]

References

  1. Selective expression of cholesterol metabolism genes in normal CD34(+)CD38(-) cells with a heterogeneous expression pattern in AML cells. Peeters, S.D., van der Kolk, D.M., de Haan, G., Bystrykh, L., Kuipers, F., de Vries, E.G., Vellenga, E. Exp. Hematol. (2006) [Pubmed]
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