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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Properties of a trifluoroleucine-resistant mutant of Saccharomyces cerevisiae.

We characterized a trifluoroleucine-resistant mutant of Saccharomyces cerevisiae, TFL20, that has a mutation in the LEU4 gene. We monitored the concentration of extracellular i-AmOH and intracellular amino acids, and compared the ratios of gene expression in TFL20 with the wild-type strain, K30. We found that the LEU1, LEU2, and BAT1 genes were up-regulated in TFL20 for metabolism, and that TFL20 simultaneously produced as much i-AmOH and leucine as K30 does.[1]


  1. Properties of a trifluoroleucine-resistant mutant of Saccharomyces cerevisiae. Oba, T., Yamamoto, Y., Nomiyama, S., Suenaga, H., Muta, S., Tashiro, K., Kuhara, S. Biosci. Biotechnol. Biochem. (2006) [Pubmed]
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