The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Genetic manipulation of a primary metabolic pathway for L: -ornithine production in Escherichia coli.

Metabolic engineering has been used to improve L: -ornithine biosynthesis in Escherichia coli W3110. L: -Ornithine production increased from 0.3 to 3.2 mg/g (dry cell weight) when the primary L: -ornithine biosynthetic pathway was optimized by disrupting the pathway transcription repressor, thereby increasing the expression of the genes involved in the pathway, and by preventing conversion of L: -ornithine into citrulline. When a feedback-resistant N-acetylglutamate synthetase gene (argA214) was placed under the control of the arabinose-inducible promoter, either in the chromosome or on a multicopy plasmid in the cell, the combination of overexpression of argA214 with an argF argI argR triple knockout mutation had an additive effect on L: -ornithine production but only when exogenous glutamate was present. When speF (which encodes ornithine decarboxylase) and proB (which encodes gamma-glutamyl kinase) were inactivated to prevent the conversion of L: -ornithine to putrescine and to block the biosynthesis of a side branch of L: -ornithine, respectively, L: -ornithine production was further enhanced approxi. 140% from 5.5 to 13.2 mg/g (dry cell weight).[1]


WikiGenes - Universities