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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Bacterial components regulate the expression of Toll-like receptor 4 on human mast cells.

OBJECTIVES AND DESIGN: The aim of this study was to investigate whether the exposure of mast cells (MCs) to bacterial components affects the expression of Toll-like receptor (TLR) 4, and to elucidate the behavior of MCs during the early response to infection. MATERIALS: Two human MC lines, HMC-1 and LAD2, were employed. Messenger RNA expression was observed by RT and real-time PCR. TLR4 expression was determined by Western blotting. TNF-alpha secretion was analyzed with ELISA. The degranulation ratio was measured with betahexosaminidase assay. RESULTS: Although bacterial components increased TLR4 mRNA, only lipopolysaccharide (LPS) augmented the TLR4 protein expression. LAD2 pre-treated with LPS for 8 h resulted in 2-fold increased TNF-alpha secretion on LPS restimulation. CONCLUSION: These results suggest that the exposure of MCs to LPS may reinforce the innate immune system due to up-regulation of MC TLR4, followed by increased TNF-alpha release.[1]

References

  1. Bacterial components regulate the expression of Toll-like receptor 4 on human mast cells. Kubo, Y., Fukuishi, N., Yoshioka, M., Kawasoe, Y., Iriguchi, S., Imajo, N., Yasui, Y., Matsui, N., Akagi, M. Inflamm. Res. (2007) [Pubmed]
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