Effect of vitamin D deficiency on D-glucose and citrate transport in rat renal basolateral membrane vesicles.
We had previously reported that feeding rats on Steenbock and Black's rickets-inducing diet markedly influences the metabolic picture of the kidney and the transmembrane transport systems of D-glucose and citrate in renal brush-border membrane vesicles. We have now studied D-glucose and citrate transport into basolateral membrane vesicles prepared from kidney cortex of control and rachitic rats and the effect of 1,25-dihydroxyvitamin D3 on these transport systems was also investigated. D-glucose and citrate uptake, determined in the presence of a Na(+)-gradient, was lowered in rachitic animals and 1,25-dihydroxyvitamin D3 administration proved to be ineffective in restoring normal values. Citrate transport, determined in the presence of a K(+)-gradient, was not influenced by both rickets and 1,25-dihydroxyvitamin D3 supply. The in vitro addition to vesicle preparations of calcium or phosphate or citrate or 1,25-dihydroxyvitamin D3 did not show a selective influence on D-glucose and citrate uptake.[1]References
- Effect of vitamin D deficiency on D-glucose and citrate transport in rat renal basolateral membrane vesicles. Stio, M., Iantomasi, T., Marraccini, P., Vincenzini, M.T., Treves, C. Biochem. Int. (1991) [Pubmed]
Annotations and hyperlinks in this abstract are from individual authors of WikiGenes or automatically generated by the WikiGenes Data Mining Engine. The abstract is from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenesOpen Access LicencePrivacy PolicyTerms of Useapsburg
