The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Beta-tubulin and benzimidazole resistance in the sheep nematode Haemonchus contortus.

We have compared benzimidazole (BZ) susceptible (s) and resistant (R) strains of Haemonchus contortus from sheep by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), Western blotting and ELISA techniques. The S strain bound more drug per mg protein than the R strain. BZ binding could be resolved into high-affinity and low-affinity binding. Low-affinity binding in parasite preparations devoid of tubulin was observed, but high-affinity binding occurred only in preparations containing tubulin. Resistance was associated with a decrease in the high affinity component. The S and R strains were shown by ELISA to contain similar total amounts of tubulin. By 2-D PAGE, the beta-tubulin isoform pattern of the S strain was different from that of the R strain, but the alpha-tubulin isoform patterns of the 2 strains were similar. BZ resistance was associated with a decrease in high-affinity BZ binding to tubulin and an alteration in beta-tubulin isoform pattern.[1]

References

  1. Beta-tubulin and benzimidazole resistance in the sheep nematode Haemonchus contortus. Lubega, G.W., Prichard, R.K. Mol. Biochem. Parasitol. (1991) [Pubmed]
 
WikiGenes - Universities