Disease relevance of tubulin

• Proteolytic digestion of 125I-ASA-BZ-labeled tubulin with Staphylococcus aureus V8 proteinase revealed one major peptide with an apparent molecular mass of 3.5 kDa [1].

High impact information on tubulin

• Like tubulin, SAS-4 is incorporated into centrioles during their duplication and remains stably associated thereafter [2].
• In fact, C. elegans phb-2(ad2154) was also found to be resistant to numerous other drugs that bind tubulin and to camptothecin, yet this mutant was sensitive to nocodazole and phalloidin [3].
• To find out whether cytoskeleton changes occurred during feeding cell development, transcriptional activity of actin (ACT) and tubulin genes and organization of the ACT filaments and of the microtubules (MTs) were analyzed in situ [4].
• The tubulin gene family, which includes alpha-,beta-, and gamma-tubulin subfamilies, is composed of highly conserved proteins which are the principle structural and functional components of eukaryotic microtubules [5].
• We are interested in (1) establishing when in eukaryotic evolution the duplications leading to paralogous alpha, beta, and gamma subfamilies occurred and (2) the possible utility of tubulin sequences in reconstructing organismal phylogeny [5].

Biological context of tubulin

• Expression of a reporter gene under the control of the C. elegans cct-1 promoter is found to be mainly restricted to neuronal and muscle tissues, an observation which is consistent with the participation of CCT in actin and tubulin folding [6].

Anatomical context of tubulin

• To examine the plant cytoskeleton during feeding cell development, transcriptional activity of actin and tubulin genes and organization of the actin filaments and of the microtubules were analyzed in situ [7].
• Microtubules reconstituted from tubulin and microtubule-associated proteins are nearly as rigid (55 microm) as the cores [8].
• Both the actin and tubulin cytoskeletons were strongly disrupted in syncytia and mitotic figures were never observed [4].

Analytical, diagnostic and therapeutic context of tubulin

• Affinity chromatography, using monoclonal antibodies anti-alpha and anti-beta-tubulin fixed to activated Sepharosa 4B-CNBr, yields a tubulin purity of 15% [9].

References