Disease relevance of Haemonchus

• Expression of cloned beta-tubulin genes of Haemonchus contortus in Escherichia coli: interaction of recombinant beta-tubulin with native tubulin and mebendazole [1].
• P-glycoprotein (P-gp), an ATP-binding transporter protein associated with multidrug resistance in mammals, protozoa and the nematode, Haemonchus contortus, might play important roles in the development of IVM resistance and/or in the tolerance of adult O. volvulus [2].
• The influence of daily doses of 0.05-0.1 mg fenbendazole/kg body weight on egg development was tested in twenty-seven lambs artificially infected with Haemonchus contortus plus Trichostrongylus colubriformis or Ostertagia circumcincta, as well as in two additional sheep naturally infected with Nematodirus spp [3].

High impact information on Haemonchus

• Haemonchus contortus GA1 antigens: related, phospholipase C-sensitive, apical gut membrane proteins encoded as a polyprotein and released from the nematode during infection [4].
• Latrophilin-like receptor from the parasitic nematode Haemonchus contortus as target for the anthelmintic depsipeptide PF1022A [5].
• Cathepsin B-like cysteine proteases confer intestinal cysteine protease activity in Haemonchus contortus [6].
• Di- and tetra-oligopeptides were used as fluorogenic substrates and irreversible/competitive inhibitors to establish CBL functions in the intestine of the parasitic nematode Haemonchus contortus [6].
• A novel mammalian galectin cDNA (ovgal11) was isolated by representational difference analysis from sheep stomach (abomasal) tissue infected with the nematode parasite, Haemonchus contortus [7].

Chemical compound and disease context of Haemonchus

• Lambs severely parasitized by Haemonchus, Ostertagia, Trichostrongylus, Cooperia, Oesophagostomum, and Nematodirus were treated with fenbendazole (methyl 5-(phenylthio)-2-benzimidazolecarbamate) given at 3 dose levels: 3.5, 5.0, and 7.5 mg/kg of body weight [8].

Biological context of Haemonchus

• Beta-tubulin genes from the parasitic nematode Haemonchus contortus modulate drug resistance in Caenorhabditis elegans [9].
• In an effort to increase our understanding of this system, we cloned and characterized a novel cDNA (5HT1Hc) from the parasitic nematode Haemonchus contortus that has high amino acid sequence homology with known G-protein coupled 5HT1-receptors from invertebrates and vertebrates [10].
• The organization and expression of a putative serine/threonine kinase gene (designated hcstk), proposed to relate to a conserved eukaryotic signal transduction pathway, was characterized for the socio-economically important pathogen Haemonchus contortus (Nematoda) [11].
• A cDNA encoding a beta-galactoside-binding lectin (galectin) was identified by immunoscreening a Haemonchus contortus cDNA library with antisera from lambs vaccinated with a membrane protein complex (H-gal-GP) derived from the parasites' gut [12].
• Levamisole binding sites in Haemonchus contortus [13].

Anatomical context of Haemonchus

• Pregnant ewes were immunised with a fraction highly enriched in the membrane glycoprotein antigen H11, isolated from the intestinal brush border of adult Haemonchus contortus [14].
• Substantial protection against the economically important nematode Haemonchus contortus has been achieved by immunizing sheep with a glycoprotein fraction isolated from the intestinal membranes of this parasite [15].
• We have identified and characterised a homologue of the C. elegans GATA transcription factor elt-2, a central regulator of endoderm development, from the parasitic nematode Haemonchus contortus [16].
• Helper T cell lines recognizing antigen in the context of MHC Class II molecules were generated from peripheral blood mononuclear cells (PBMC) of sheep undergoing a primary or secondary infection with the nematode parasite Haemonchus contortus [17].
• Three tubulin isotypes from the parasitic nematode Haemonchus contortus were individually expressed in Escherichia coli, purified, and induced to polymerize into microtubules in the absence of microtubule-associated proteins [18].

Associations of Haemonchus with chemical compounds

• Structural studies on the N-linked oligosaccharides of Haemonchus contortus, an economically important nematode that parasitizes domestic ruminants, have revealed core fucosylation of a type not previously observed in any eukaryotic glycoprotein [19].
• Genetic variability of the beta-tubulin genes in benzimidazole-susceptible and -resistant strains of Haemonchus contortus [20].
• Binding of hematin by a new class of glutathione transferase from the blood-feeding parasitic nematode Haemonchus contortus [21].
• Two alleles of the HG1 gene, which encodes a putative GABA receptor alpha/gamma subunit, were isolated from Haemonchus contortus [22].
• Specific interaction of benzimidazole anthelmintics with tubulin from developing stages of thiabendazole-susceptible and -resistant Haemonchus contortus [23].

Gene context of Haemonchus

• Detection of P-glycoprotein-mediated multidrug resistance against anthelmintics in Haemonchus contortus using anti-human mdr1 monoclonal antibodies [24].
• Actin isoforms in the parasitic nematode Haemonchus contortus [25].
• Haemonchus contortus: cloning and functional expression of a cDNA encoding ornithine decarboxylase and development of a screen for inhibitors [26].
• Therefore, the molecular mechanism of levamisole resistance in the parasitic nematode Haemonchus contortus was studied by isolating and characterising cDNA clones encoding a putative ligand binding nicotinic acetylcholine receptor subunit, HCAl, of two susceptible and one levamisole resistant population [27].
• Characterization of an acetylcholine receptor gene of Haemonchus contortus in relation to levamisole resistance [27].

Analytical, diagnostic and therapeutic context of Haemonchus

• The response of sheep to parenteral vaccination and immunizing infections against the abomasal nematode, Haemonchus contortus [28].
• Molecular cloning and primary sequence of a cysteine protease expressed by Haemonchus contortus adult worms [29].
• Previous work has shown that a protein extract enriched for cysteine protease activity (TSBP) prepared from adult Haemonchus contortus using thiol sepharose affinity chromatography confers substantial protection against a single challenge infection [30].
• In addition, a generic PCR approach was adopted in order to identify the predominant cysteine proteinases in a UK strain of Haemonchus [31].
• The effect of immunosuppression with the glucocorticosteroid, dexamethasone, on the susceptibility to Haemonchus contortus infection of a highly resistant Merino genotype was investigated [32].

References