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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Cloned endothelial cells from fetal bovine bone.

Primary cell cultures from fetal bovine sternum were developed in Coon's modified Ham's F-12 medium containing 10% Nu-Serum, 1% Ultroser-G, and 200 mg of galactose per liter. Clones were obtained by colony isolation; one clone, BBE-1, was selected for characterization. BBE-1 cells exhibited typical endothelial morphology by light and electron microscopy and immunofluorescence for factor VIII-related antigen throughout their life span of 8 months. The cells showed mitogenic responses to endothelial cell growth factor, basic fibroblast growth factor, insulin-like growth factor types I and II, platelet-derived growth factor, ascorbic acid, and progesterone. Parathyroid hormone stimulated intracellular accumulation of cAMP in BBE-1 cells but not in endothelial cells from two other tissues. These clonal cells provide a useful system for studies on bone vasculature, including its interactions with other bone cells.[1]

References

  1. Cloned endothelial cells from fetal bovine bone. Streeten, E.A., Ornberg, R., Curcio, F., Sakaguchi, K., Marx, S., Aurbach, G.D., Brandi, M.L. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
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