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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Cloned endothelial cells from fetal bovine bone.

Primary cell cultures from fetal bovine sternum were developed in Coon's modified Ham's F-12 medium containing 10% Nu-Serum, 1% Ultroser-G, and 200 mg of galactose per liter. Clones were obtained by colony isolation; one clone, BBE-1, was selected for characterization. BBE-1 cells exhibited typical endothelial morphology by light and electron microscopy and immunofluorescence for factor VIII-related antigen throughout their life span of 8 months. The cells showed mitogenic responses to endothelial cell growth factor, basic fibroblast growth factor, insulin-like growth factor types I and II, platelet-derived growth factor, ascorbic acid, and progesterone. Parathyroid hormone stimulated intracellular accumulation of cAMP in BBE-1 cells but not in endothelial cells from two other tissues. These clonal cells provide a useful system for studies on bone vasculature, including its interactions with other bone cells.[1]


  1. Cloned endothelial cells from fetal bovine bone. Streeten, E.A., Ornberg, R., Curcio, F., Sakaguchi, K., Marx, S., Aurbach, G.D., Brandi, M.L. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
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