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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Autoradiographic demonstration of gastrin-releasing peptide-binding sites in the rat gastric mucosa.

The location of [125I]iodotyrosyl gastrin-releasing peptide-binding sites in the rat fundic mucosa was studied. Peptide specificity was demonstrated by competitive binding studies using the addition of a large amount of cold gastrin-releasing peptide or substance P. Autoradiography of the stomach tissue was carried out by freeze-drying, embedding in Epon, wet-sectioning with ethylene glycol, and dry-mounting the emulsion film by the wire-loop method to prevent loss of the labeled substance. Specific binding sites of gastrin-releasing peptide were found on D cells, surface mucus cells, and parietal cells, whereas few binding sites were seen on the chief or mucus neck cells.[1]

References

  1. Autoradiographic demonstration of gastrin-releasing peptide-binding sites in the rat gastric mucosa. Nakamura, M., Oda, M., Kaneko, K., Akaiwa, Y., Tsukada, N., Komatsu, H., Tsuchiya, M. Gastroenterology (1988) [Pubmed]
 
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