The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

Poly-N-acetyllactosamine alterations of Thy-1 glycoprotein in lymphocyte differentiation.

Developmental changes in murine lymphocyte Thy-1 include both quantitative and qualitative alterations involving N-linked oligosaccharides. Comparison of immature with mature T-cells has shown that the oligosaccharides of Thy-1 are characterized by an increase in the number of sialic acid residues responsible for the acidic pI of peripheral T-cell Thy-1, and a decrease in those oligosaccharides responsible for Mr heterogeneity of thymocyte Thy-1. The research reported here suggests that the basis of the large Mr heterogeneity in Thy-1 of immature T-cells is the presence of repeating N-acetyllactosamine (R'Gal beta 1,4GlcNAc beta 1,3R") units in the oligosaccharide portion of the molecule. Lymphocytes were surface iodinated and 125I-thy-1 was purified by immunoprecipitation and preparative nonequilibrium pH gradient electrophoresis. The minimal Mr of unglycosylated Thy-1 after endoglycosidase F digestion was 15,000-16,000. Digestion of Thy-1 with endo-beta-galactosidase suggested that the complex type N-linked glycans in thymocytes, but not in lymph node T-cells, contained increased levels of polylactosamine. The presence of polylactosamine was confirmed by binding to a Datura stramonium lectin column which retarded and bound approx. 50% of thymocyte Thy-1 and only about 18% of lymph node T-cell Thy-1. Affinity chromatography using anti-i antibody immobilized on agarose beads indicated that the polylactosamine is probably present in a predominantly linear form. Since alterations of polylactosamine structures have been implicated in development and transformation in several systems, the present results suggest an important role for these glycans in immune-cell differentiation.[1]

References

  1. Poly-N-acetyllactosamine alterations of Thy-1 glycoprotein in lymphocyte differentiation. Morrison, M.H., Lynch, R.A., Esselman, W.J. Mol. Immunol. (1986) [Pubmed]
 
WikiGenes - Universities