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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Studies of monokines as mediators of the acute phase response: effects on sialyltransferase, albumin, alpha 1-acid glycoprotein and beta-N-acetylhexosaminidase.

Rat peritoneal leukocytes (PEC) were fractionated on Percoll gradients to prepare populations of monocytes/lymphocytes and polymorphonuclear leukocytes; adherent (monocyte enriched) and non-adherent (lymphocytes/polymorphonuclear leukocytes) cells were also isolated from PEC. Cytokines were prepared from PEC and subfractions and injected into rats to induce the acute phase reactants serum alpha 1-acid glycoprotein and sialyltransferase; negative acute phase parameters serum albumin and liver hexosaminidase were also assayed. Monocyte derived cytokines (monokines) mimicked the acute phase response of all four parameters in vivo. The sialyltransferase isoenzyme that responded to monokine was identified as the Gal beta 1----4GlcNAc alpha 2----6 isoenzyme.[1]

References

  1. Studies of monokines as mediators of the acute phase response: effects on sialyltransferase, albumin, alpha 1-acid glycoprotein and beta-N-acetylhexosaminidase. Woloski, B.M., Gospodarek, E., Jamieson, J.C. Biochem. Biophys. Res. Commun. (1985) [Pubmed]
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