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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Lectin-dependent attachment of Actinomyces naeslundii to receptors on epithelial cells.

The adherence of Actinomyces naeslundii WVU45 to monolayer cultures of human epithelial cell lines was mediated by the lactose-sensitive fimbriae (type 2) of strain WVU45. The attachment of Actinomyces viscosus T14V, which has both types 1 and 2 fimbriae, was approximately half that of A. naeslundii, and only minimal attachment of A. naeslundii and A. viscosus mutants lacking type 2 fimbriae was detected. The adherence of strain WVU45 was enhanced two- to threefold by neuraminidase treatment of the epithelial cells. The Fab fragments of antibodies which recognize the type 2 fimbriae inhibited the adherence of A. naeslundii WVU45 to the epithelial cells. The bacterial interaction with epithelial cells was inhibited by lactose, methyl-beta-D-galactoside, and N-acetyl-D-galactosamine, but not by methyl-alpha-D-galactoside, cellobiose, N-acetyl-D-glucosamine, L-fucose, or D-mannose. To further characterize the epithelial cell receptors for the bacterial lectin, we utilized several plant and invertebrate lectins as potential inhibitors of bacterial adherence. Lectins from Bauhinia purpurea and Arachis hypogaea which recognize N-acetyl-D-galactosamine, D-galactose, and D-galactose-beta-(1----3)-N-acetyl-D-galactosamine inhibited bacterial attachment, and binding of these lectins to epithelial cells was enhanced by the addition of neuraminidase. Lectins reacting with alpha-linked D-galactose, alpha-linked N-acetyl-D-galactosamine, D-mannose, or sialic acid were not inhibitory. Under similar assay conditions, adherence of a mannose-sensitive strain of Escherichia coli was inhibited by concanavalin A but not by the lectin from Bauhinia purpurea. These results indicate that certain plant lectins have specificities similar to that of the actinomyces fimbrial lectin and are, therefore, useful probes for identifying receptors on epithelial cells for certain bacteria.[1]


  1. Lectin-dependent attachment of Actinomyces naeslundii to receptors on epithelial cells. Brennan, M.J., Cisar, J.O., Vatter, A.E., Sandberg, A.L. Infect. Immun. (1984) [Pubmed]
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