Epstein-Barr virus nuclear antigen-positive nasopharyngeal hybrid cells.
An epithelial-like hybrid cell line was established by cell fusion of 8-azahypoxanthine-resistant epithelial cells (Ad-AH) with lymphoblastoid cells (A2L), derived from the human nasopharynx. The nasopharyngeal hybrid cells, designated as A2L/AH, were Epstein-Barr virus (EBV)-associated nuclear antigen (EBNA)-positive by the anticomplement immunofluorescence method. Furthermore, the treatment of the hybrid cells with 5-Iodo-2' -deoxyuridine (IUDR) induced early antigen (EA) and viral capsid antigens (VCA), while the treatment of nonproducer lymphoblastoid cells, A2L, induced EA but not VCA. The appearance of IUDR-induced VCA in the hybrid cells suggests that some factor produced by the Ad-AH cells might neutralize a repressed state of VCA and thus activate these antigens with the treatment of IUDR. These established nasopharyngeal hybrid cells might be useful for studies of in vitro nasopharyngeal carcinoma ( NPC) since no EBV-carrying NPC cell lines have been established.[1]References
- Epstein-Barr virus nuclear antigen-positive nasopharyngeal hybrid cells. Takimoto, T., Furukawa, M., Hatano, M., Umeda, R. The Annals of otology, rhinology, and laryngology. (1984) [Pubmed]
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