The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Liquid chromatographic determination of tenuazonic acids in tomato paste.

Tenuazonic acid is a mycotoxin produced by Alternaria alternata (A. tenuis) occurring in isolates from tomato. Several liquid chromatographic (LC) systems were compared for suitability in analyzing tomato pastes for tenuazonic acid. Tenuazonic acid was extracted from tomato paste with methanol and n-hexane and was partitioned into methylene chloride from the aqueous methanol following acidification. It was further partitioned into 5% NaHCO3, acidified, and reextracted with methylene chloride. LC separation of tenuazonic acid from interferences was best on a C12-dien (4-dodecyldiethylenetriamine) loaded C18 reverse phase column with a mobile phase containing C12-dien, Zn(II), and ammonium acetate, or on a tetraalkylammonium anion exchange column at pH 7. 5. Two isomeric tenuazonic acids could be partially separated in the C12-dien/metal system using acetonitrile in the mobile phase. Amounts of standard tenuazonic acid as low as 2-5 nm. Recoveries of tenuazonic acid or isotenuazonic acid added to tomato paste at levels of 0.4-10 micrograms/g were 67-94%. Small amounts of tenuazonic acid were detected in several commercial tomato pastes at levels of 0.01-0.1 micrograms/g. Gas liquid chromatography/mass spectrometric (GLC/MS) single ion monitoring showed that both isomers were present.[1]

References

  1. Liquid chromatographic determination of tenuazonic acids in tomato paste. Scott, P.M., Kanhere, S.R. Journal - Association of Official Analytical Chemists. (1980) [Pubmed]
 
WikiGenes - Universities