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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Expression and secretion of an Arthrobacter dextranase in the oral bacterium Streptococcus gordonii.

We have constructed a plasmid to express and secrete dextranase in the oral bacterium Streptococcus gordonii. The dextranase gene from Arthrobacter sp. strain CB-8 was linked to a promoter and a DNA sequence encoding the signal peptide of Streptococcus downei glucosyltransferase I (gtfI) followed by the Escherichia coli rrnBt1t2 terminator and inserted in the shuttle vector pVA838. S. gordonii transformed with this plasmid (pMNK-4) expressed and secreted mature Arthrobacter dextranase. The transformant was found to repress the firm adherence of water-insoluble glucan in a coculture experiment with cariogenic bacteria, Streptococcus sobrinus, in the presence of sucrose. Such genetically engineered oral bacteria could provide a therapy to prevent dental caries.[1]

References

  1. Expression and secretion of an Arthrobacter dextranase in the oral bacterium Streptococcus gordonii. Kubo, S., Kubota, H., Ohnishi, Y., Morita, T., Matsuya, T., Matsushiro, A. Infect. Immun. (1993) [Pubmed]
 
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