The presence of the 50-kDa subunit of dynactin complex in the nerve growth cone.
Membrane proteins in growth cone-enriched and growth cone-non-enriched fractions prepared from neonatal mouse brain were separated by lectin-affinity and ion exchange chromatographies, 2D-PAGE, and SDS-PAGE. Partial amino acid sequences of the proteins concentrated in the growth cone-enriched fraction were determined. We found that one such protein, gmp23-48k, corresponds to the 50-kDa subunit (p50) of the dynactin complex. An antibody raised against gmp23-48k strongly reacted with growth cones of differentiated neuronal precursor cells. Immunoblot analyses revealed that gmp23-48k was present both in membrane and in soluble fractions of neonatal brain. However, the amount of gmp23-48k in the membrane fraction greatly decreased in adult brain. These results suggest a special role of membrane-associated gmp23-48k/p50 in synapse formation during brain development.[1]References
- The presence of the 50-kDa subunit of dynactin complex in the nerve growth cone. Abe, T.K., Tanaka, H., Iwanaga, T., Odani, S., Kuwano, R. Biochem. Biophys. Res. Commun. (1997) [Pubmed]
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