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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

Members of the glutathione S-transferase gene family are antigens in autoimmune hepatitis.

BACKGROUND & AIMS: Autoimmmune hepatitis (AIH), a chronic liver disorder, can be classified into two subtypes on the basis of the specificities of circulating autoantibodies. Type I AIH is defined by antibodies to nuclear and/or smooth muscle antigens (SMA), and type II is characterized by antibodies to cytochrome P450IID6. There is an additional type of AIH characterized by antibodies to a cytosolic soluble liver antigen ( SLA), which can occur alone or in combination with antinuclear antibodies and SMA. The aim of this study was to identify the reactive antigen in SLA, a heterogenous cytosolic fraction consisting of at least 100 extremely soluble proteins. METHODS: Sera from 31 patients with AIH reacting with SLA and from 30 disease controls were tested. The immunoreactive antigens were determined using immunoprecipitation and immunoblotting after one- and two-dimensional polyacrylamide gel electrophoresis. The antigens were identified by microsequencing of the corresponding protein spots. RESULTS: Twenty-five of 31 anti- SLA-positive sera (80, 7%) reacted with a set of proteins ranging from 25 to 27 kilodaltons that were identified as three subunits of glutathione S-transferases: Ya, Yb1, and Yc. CONCLUSIONS: Glutathione S-transferase subunit proteins represent the major autoantigen in anti- SLA-positive AIH. This new finding permits the establishment of standardized immunoassays for routine diagnosis.[1]

References

  1. Members of the glutathione S-transferase gene family are antigens in autoimmune hepatitis. Wesierska-Gadek, J., Grimm, R., Hitchman, E., Penner, E. Gastroenterology (1998) [Pubmed]
 
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