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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Hyaluronan in the interphotoreceptor matrix of the eye: species differences in content, distribution, ligand binding and degradation.

Hyaluronan (HA) distribution in the posterior eye wall from the vitreous through the sclera, with special consideration to localization in the retina and interphotoreceptor matrix (IPM), was evaluated in human, bovine, guinea pig, dog, rat and mouse tissues using a specific probe for HA (bHABC, biotinylated hyaluronan binding complex). The sclera, some regions of the choroid and vitreous body was positive for HA, as was the basal lamina of the retina (inner limiting membrane). bHABC binding was detected in the IPM of all species studied except the mouse. Predigestion with Streptomyces hyaluronidase for 3 hr before bHABC application eliminated binding in the vitreous, choroid, sclera and basal lamina of the retina, but did not eliminate bHABC binding in the IPM. In tissues from all species studied, incubation for 6 hr with hyaluronidase eliminated bHABC binding in the IPM, except for two human samples. In these two human samples, HA specific binding in the IPM persisted even after 24 hr enzyme treatment. bHABC failed to bind to any tissue layer when bHABC was preincubated with hyaluronan oligosaccharides before application. The resistance of the IPM HA to hyaluronidase digestion may reflect extensive coverage of HA binding sites by ligands present in this compartment which hinder enzyme access. The absence of bHABC binding to the IPM when the probe is preincubated with HA oligosaccharides indicates that the binding reflects specific interaction with HA. We conclude that, with the exception of the mouse, HA is a prominent constituent of the IPM, where it may serve to organize the matrix by functioning as a basic scaffold to which other macromolecules in the insoluble IPM are attached.[1]


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