TGF-beta 3 inhibits the increased gene expressions of pulmonary surfactant proteins induced by dexamethasone in fetal rat lung in vitro.
OBJECTIVE: To determine the effects of transforming growth factor-beta 3 (TGF-beta 3) on expressions of surfactant proteins (SPs). METHODS: Northern blot hybridization and reverse transcriptase polymerase chain reaction (RT-PCR) were employed to determine the SP-A, SP-B and SP-C mRNA levels of cultured lung explants and type II cells from fetal rat lungs. RESULTS: TGF-beta 3 alone did not affect the expressions of SPs either in explants or in type II cells. However, TGF-beta 3 did inhibit the increase of SP-B, SP-C mRNA levels caused by dexamethasone (100 nmol/L), in a dose-dependent manner. Furthermore, TGF-beta 3 had no direct inhibition on the increase of SP-B, SP-C mRNA induced by dexamethasone in type II cells, but had an indirect inhibitory effect mediated by fibroblasts. CONCLUSION: TGF-beta 3 plays an important inhibitory role in the development of pulmonary surfactant proteins. The present study may provide a possible explanation for the unideal effect of glucocorticoids in the prevention of neonatal respiratory distress syndrome.[1]References
- TGF-beta 3 inhibits the increased gene expressions of pulmonary surfactant proteins induced by dexamethasone in fetal rat lung in vitro. Wang, X., Jin, Q., Xu, J., Shen, W., Wang, J., Post, M. Chin. Med. J. (1997) [Pubmed]
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