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Chemical Compound Review:

AC1Q1VLZ sodium; oxygen(-2) anion; tungsten;...

Synonyms: AR-1L5349, AR-1L5350, LS-109052, AC1L4QP3, I14-114761, ...

Fishbein, W.N. et al., Callahan, G.N. et al., Durrington, P.N., Avogaro, P. et al., Telford, J.N. et al., et al.

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Disease relevance of EINECS 257-132-9

With conventional negative staining procedures using neutral sodium phosphotungstate, both murine mammary tumor virus and murine leukemia virus appeared in head-and-tail forms, with a peak head diameter of 122 and 130 nm, respectively [1].

High impact information on EINECS 257-132-9

Electron microscopy of native and dimethyl-suberimidate crosslinked rabbit muscle phosphofructokinase (EC 2.7.1.11; ATP:D-fructose-6-phosphate 1-phosphotransferase) has been carried out using negative staining with sodium phosphotungstate [2].
Sera obtained from normal B10.BR mice were shown to inhibit selectively a specific anti-Ia alloantiserum.Partial purification of the Ia antigenic activity was accomplished by isolation of the high density lipoproteins from these sera by fractional precipitation with sodium phosphotungstate and MgCL2 [3].
Effect of reagent pH on determination of high-density lipoprotein cholesterol by precipitation with sodium phosphotungstate-magnesium [4].
Both ammonium molybdate and sodium phosphotungstate were able to show the differences in Fim 3 stored at pH 7.2 and pH 9.5 [5].
Precipitation of serum low and very low density lipoproteins by sodium phosphotungstate and magnesium chloride results in complete recovery of lecithin-cholesterol acyltransferase activity in the supernatant [6].

Associations of EINECS 257-132-9 with other chemical compounds

Serum high density lipoprotein (HDL) cholesterol was measured in non-diabetic and diabetic men and women by ultracentrifugation, heparin-manganese chloride precipitation (heparin/Mn++), and sodium phosphotungstate-magnesium chloride precipitation (PT/Mg++) [7].

Gene context of EINECS 257-132-9

An electroimmunoassay of apolipoprotein E (Apo E) in total human plasma and in the supernatant following precipitation with sodium phosphotungstate in the presence of MgCl2 of the apolipoprotein B (Apo B)-containing lipoproteins is described [8].

Analytical, diagnostic and therapeutic context of EINECS 257-132-9

Samples were clarified by centrifugation, pelleted in the EM-90 rotor directly to Formvar-coated copper grids, and strained with 1.5% sodium phosphotungstate [9].
Electron microscopy, with sodium phosphotungstate as negative stain, has been carried out on purified jackbean urease prepared at three levels of quaternary structure: (a) A1 urease, Mr = 240 000, S20,W = 11.5 S (b) alpha urease, Mr = 480 000, S20,w = 18.3 S (c) polymers of alpha urease above the tetramer stage [10].

References

The morphology of murine oncornaviruses following different methods of preparation for electron microscopy. Sarkar, N.H., Manthey, W.J., Sheffield, J.B. Cancer Res. (1975) [Pubmed]
Electron microscope study of native and crosslinked rabbit muscle phosphofructokinase. Telford, J.N., Lad, P.M., Hammes, G.G. Proc. Natl. Acad. Sci. U.S.A. (1975) [Pubmed]
Characterization of Ia antigens in mouse serum. Callahan, G.N., Ferrone, S., Poulik, M.D., Reisfeld, R.A., Klein, J. J. Immunol. (1976) [Pubmed]
Effect of reagent pH on determination of high-density lipoprotein cholesterol by precipitation with sodium phosphotungstate-magnesium. Grove, T.H. Clin. Chem. (1979) [Pubmed]
Negative staining can cause clumping of Bordetella pertussis fimbriae. Hamilton, R.C., Bennet, J., Drane, D., Pietrzykowski, E., Seddon, F., Stefancic, A., Cox, J. Micron (1994) [Pubmed]
A lipoprotein independent assay for human serum lecithin-cholesterol acyltransferase. Morin, R.J., Piran, U. Clin. Chim. Acta (1981) [Pubmed]
A comparison of three methods of measuring serum high density lipoprotein cholesterol in diabetics and non-diabetics. Durrington, P.N. Ann. Clin. Biochem. (1980) [Pubmed]
Distribution and concentration of apolipoprotein E in high and low density lipoproteins. Avogaro, P., Bittolo Bon, G., Cazzolato, G., Pontoglio, E., Kostner, G.M. La Ricerca in clinica e in laboratorio. (1983) [Pubmed]
Improved detection of viruses by electron microscopy after direct ultracentrifuge preparation of specimens. Hammond, G.W., Hazelton, P.R., Chuang, I., Klisko, B. J. Clin. Microbiol. (1981) [Pubmed]
Electron microscopy of negatively stained jackbean urease at three levels of quaternary structure, and comparison with hydrodynamic studies. Fishbein, W.N., Engler, W.F., Griffin, J.L., Scurzl, W., Bahr, G.F. Eur. J. Biochem. (1977) [Pubmed]

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