Disease relevance of Fractional Precipitation

• Cathepsin B (EC 3.4.22.1) was purified 746-fold with a 21% recovery from bovine brain by autolysis, fractional precipitation with acetone, carboxy-methyl-Sephadex chromatography, affinity chromatography on a cystamine containing column and gel filtration chromatography [1].

High impact information on Fractional Precipitation

• H1t was isolated in homogeneous form by extraction of whole tissue with 0.75 M perchloric acid, fractional precipitation with trichloroacetic acid, and two cycles of chromatography over a cation exchange resin (Bio Rex 70) [2].
• Sera obtained from normal B10.BR mice were shown to inhibit selectively a specific anti-Ia alloantiserum.Partial purification of the Ia antigenic activity was accomplished by isolation of the high density lipoproteins from these sera by fractional precipitation with sodium phosphotungstate and MgCL2 [3].
• Seryl-tRNASerCmCA: ATP phosphotransferase was purified 1200-fold from bovine liver by ultracentrifugation at 150 000 X g, chromatography on DEAE-cellulose, fractional precipitation with ammonium sulfate, chromatography on hydroxyapatite, gel filtration on Sephacryl S-300 and affinity chromatography on Blue Sepharose [4].
• Subsequent purification by ammonium sulfate fractional precipitation results in negligible improvement in 9-hydroperoxide formation [5].
• Gaseous CO2 was used as an antisolvent to induce the fractional precipitation of alkaline phosphatase, insulin, lysozyme, ribonuclease, trypsin, and their mixtures from dimethylsulfoxide (DMSO) [6].

Associations of Fractional Precipitation with chemical compounds

• A combination with a preceding prepurification by a fractional precipitation with polyethylene glycol resulted in a strong affinity effect accompanied by an efficient purification during IMAP (purification factor of 11 with a yield of approximately 90%) [7].
• Immunochemical study on bakers' yeast mannan prepared by fractional precipitation with cetyltrimethylammonium bromide [8].
• The isolation of phosphomannan-protein complexes from the viable cells of yeast (Y) and mycelial (M) forms of Candida albicans NIH B-792 strain was conducted by treatment with Zymolyase-100T followed by fractional precipitation with cetyltrimethylammonium bromide [9].
• This new enzyme in the subclass 2.4.1 catalyzes the glucosylation of the 7-OH group of naringenin (4',5',7-trihydroxyflavanone) to prunin and has been purified (943-fold) by fractional precipitation with ammonium sulfate and successive chromatography on Sephadex G-100, hydroxyapatite, UDP-glucuronic acid agarose, Mono Q, and Mono P columns [10].
• If the guanidinium chloride is removed by dialysis in a single step, the enzyme reprecipitates, but if a fractional precipitation is performed by reducing the guanidinium chloride concentration by dilution, the enzyme remains in solution at 0.2 M guanidinium chloride [11].

Gene context of Fractional Precipitation

• Oxytocinase (cystine aminopeptidase) was purified from human retroplacental serum by a combination of fractional precipitation, hydroxylapatite chromatography and gel exlusion chromatography on Sephadex G-200 [12].
• Another approach to protein separation in DMSO, fractional precipitation with ethyl acetate (which does not dissolve proteins), has been verified with a mixture of bovine pancreatic chymotrypsinogen and chicken egg ovalbumin [13].
• Immunochemical study on the mannans of Candida albicans NIH A-207, NIH B-792, and J-1012 strains prepared by fractional precipitation with cetyltrimethylammonium bromide [14].
• Purification of NodD was performed by the sequential steps: polyethyleneimine treatment, ammonium sulfate fractional precipitation, and cellulose phosphate P11 chromatography [15].
• Fractional precipitation of human plasma using ethanol, followed by chromatography on S.P. Sephadex, yielded a somatomedin-enriched fraction freed from substantial amounts of inhibitory substances [16].

Analytical, diagnostic and therapeutic context of Fractional Precipitation

• 6. The enzyme in each of these two fractions was purified to the electrophoretically pure state by fractional precipitation with ammonium sulfate, column chromatographies on DEAE-cellulose, hydroxyapatite, Bio Gel A-1.5 m and affinity chromatography on heated glutinous rice [17].
• SMP 6.1 was purified from the culture supernatant by ammonium sulfate precipitation, acetone fractional precipitation, and preparative isoelectric focusing [18].
• After DEAE cellulose chromatography and purification fractional precipitation by 50% of the saturated ammonium sulfate of the extract prepared from A. vinelandii cells, a hexagonal crystalline preparation is obtained [19].
• The isolation of FTBL protein as well as the isolation of catalase involves a preliminary fractional precipitation and solution before crystallization can be achieved [20].

References